Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 4 Issue 12

Zingiber officinale (Ginger) Extract has No Effect on Kiss1 Gene Expression in the Testis and Blood but May Cause Inflammation-induced Morphological Sperm Disruptions in Wistar Rats

Izuchukwu Azuka Okafor1,2,3*, Uchenna Somtochukwu Nnamah4, Johnson Okwudili Nweke5, Jude Amechi Nnaka1, Selasie Ahiatrogah2,3 and Uche Valentine Okeke6

1Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria
2Department of Obstetrics and Gynaecology, College of Medicine, University of Ibadan, Ibadan, Nigeria
3Pan African University of Life and Earth Science Institute (Including Health and Agriculture), PAULESI, University of Ibadan, Ibadan, Nigeria
4Hematology Department, Babcock University Teaching Hospital, Ilisan Remo, Ogun State, Nigeria
5Diagnostic Laboratory Unit, Medical Centre, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
6Morbid Anatomy Department, Alex Ekwueme Federal University Teaching Hospital Abakaliki, Ebonyi State, Nigeria

*Corresponding Author: Izuchukwu Azuka Okafor, Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria.

Received: September 23, 2020; Published: November 18, 2020

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Abstract

Background: Zingiber officinale is used extensively both as a culinary spice and herbal medicine for the treatment of different ailments.

Aim: This study investigated the role of ginger in the Kiss1 gene expression in the testis of Wistar rats.

Methods: Fifteen (15) rats with an average weight of 104g were randomly divided into three (3) groups (A-C). Group A was given no treatment and served as the normal control group. Groups B and C received oral administration of 200 mg/kg and 400 mg/kg of methanolic extract of Zingiber officinale (MEZO) respectively. The extract was administered once a day for 21 days.

Results: There was no significant difference in the testicular oxidative status, LH, FSH, TT, sperm motility, sperm count, and sperm morphology across all experimental groups (p < 0.05) except for group C where a significant decrease in sperm morphology was observed compared to the control (p = 0.007). Some vascular congestions with visible aggregates of inflammatory red cells were also observed in group C testes. The relative intensity of Kiss1 gene expression observed in the testis is significantly higher than that in the blood at 200 mg/kg of MEZO (CI: -1.39 - (-0.05), p = 0.045) with a significant negative correlation between the sperm count and the relative intensity of KISS1 gene expression in the blood (r = -.882, p = 0.20).

Conclusion: MEZO does not affect the Kiss1 gene expression levels in the testis and blood of Wistar rats but shows the potential of causing sperm morphological disruptions due to testicular inflammatory response at a high dose.

Keywords: Zingiber officinale; Sperm Count; Sperm Morphology; Sperm Motility; Kiss1; Kisspeptin; Ginger; Gene Expression; Hormones; Testis; Histology; Oxidative Markers

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Citation

Citation: Izuchukwu Azuka Okafor., et al. “Zingiber officinale (Ginger) Extract has No Effect on Kiss1 Gene Expression in the Testis and Blood but May Cause Inflammation-induced Morphological Sperm Disruptions in Wistar Rats". Acta Scientific Medical Sciences 4.12 (2020): 17-25.




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