Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 4 Issue 3

Optimizing the Fermentation Conditions and Enhancing the Keratinase Production from Streptomyces coelicoflavus

Jadhav RS1, JK Oberoi2, Tejashree Rokade2, Rajesh Shingade2, Mrunal Yadav2, Tooba Momin2*

1Department of Microbiology, Vishwasrao Niak Arts, Commerce and Baba Naik Sciences, Mahavidyalaya, Shirala, India
2Department of Microbiology, Abeda Inamdar Senior College, Pune, India

*Corresponding Author: Tooba Momin, Department of Microbiology, Abeda Inamdar Senior College, Pune, India.

Received: October 12, 2020; Published: February 10, 2021

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Abstract

  Total eight soil samples were taken from Sangli district (Maharashtra) India. The potent isolates was identified by using morphological, cultural, biochemical, physiological and 16S rRNA gene sequence analysis. Keratinase production was optimized using different parameters. The 120 hrs incubation period of was observed for optimum keratinolytic protease production. Inoculum age of 10 days was found to be effective in terms of protease production and degradation. 3% inoculum size was showed optimum degradation and proteolytic activity. The agitation rate at 160 rpm was found to be optimum. Optimum enzyme production and feather degradation was found at pH 9.0. Maximum production of protease was observed at 400C. The optimum amount of keratinase was produced by Streptomyces coelicoflavus in presence of dextrose and peptone as a carbon and nitrogen source separately respectively. 1% feather concentration was found optimum keratinase production. Streptomyces coelicoflavus was enhanced feather degradation in the presence of KH2PO4.

Keywords: Streptomyces coelicoflavus, 16S rRNA, Keratinase, Optimization and Degradation.

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Citation

Citation: Tooba Momin., et al. “Optimizing the Fermentation Conditions and Enhancing the Keratinase Production from Streptomyces coelicoflavus". Acta Scientific Microbiology 4.3 (2021): 14-24.




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