Hayfa Argui1,3*, Salih Can Suner2,5, Çağdaş Deniz Periz4, Seyhan Ulusoy4, Mossadok Ben-Attia3, Ayhan Oral2, Yalçın Coşkun6 , Ayhan Oral7 and Hechmi Said1
1Materials Chemistry Laboratory, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, Tunisia
2Department of Chemistry and Chemical Processing, Lapseki Vocational School, Canakkale Onsekiz Mart University, Turkey
3Biomonitoring of the Environment Laboratory (LR01/ES14), Faculty of Sciences of Bizerte, University of Carthage, Tunisia
4Department of Biology, Faculty of Science, Suleyman Demirel University, Turkey
5Canakkale Onsekiz Mart University Application in Science and Technology and Research Laboratory, Turkey
6Canakkale Onsekiz Mart University Department of Organic Farming, Lapseki Vocational College, Turkey
77Department of Chemistry, Faculty of Art and Science, Canakkale Onsekiz Mart University 17100 Çanakkale, Turkey.
*Corresponding Author: Hayfa Argui, Materials Chemistry Laboratory, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, Tunisia.
Received: December 29, 2020 ; Published: January 28, 2021
The objective of this work was to evaluate antibacterial and antibiofilm activity of Eucalyptus camaldulensis EO-loaded PLA nanofibers. Different concentrations of E. camaldulensis EO (7.5, 10 and 15%) were incorporated into PLA nanofibers via an electrospinning technique. The nanofibers are characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic mechanical analysis (DMA). Antibacterial activity of essential oil-loaded PLA nanofibers against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Enterococcus faecalis were investigated by disc diffusion method. The antibiofilm activity of essential oil-loaded PLA nanofibers was studied against Pseudomonas aeruginosa. The results obtained from the biofilm formation test revealed that PLA essential oil inhibited the Pseudomonas aeruginosa biofilm by 9.3%, 12.8% and 12.6%, respectively. Drug releasing behavior of essential oil-loaded PLA nanofibers was investigated by UV-Visible spectroscopy. According to releasing results, as the amounts of Eucalyptus camaldulensis essential oil in PLA nanofibers increased, the releasing decreased. The essential oil-loaded PLA nanofiber successfully delivered about 160 h.
Keywords: Eucalyptus camaldulensis Dehn; Electrospinning; Nanofibers; Drug Delivery; Antibacterial Activity; Antibiofilm Activity
Citation: Hayfa Argui., et al. “Fabrication, Characterization, In Vitro Release, and Some Biological Activities of Eucalyptus Essential Oil Loaded Poly (Lactic Acid) Nanofibers”. Acta Scientific Microbiology 4.2 (2021): 54-63.
Copyright: © 2021 Hayfa Argui., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.