Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 9

Diversity of Begomoviruses Infecting Okra [Abelmoschus esculentus (L.) Moench] in Togo

Fidèle Tiendrébéogo1,2*, Ayékitan Akakpo1,2,3, Ezechiel B Tibiri1,2, Monique Soro1,2, Cheikna Zongo3, Aly Savadogo3, James Bouma Neya1,2, Kossikouma Djodji Adjata4 and Nicolas Barro5

1Laboratoire de Virologie et de Biotechnologies Végétales, INERA/CNRST, Burkina Faso
2Laboratoire Mixte International Patho-Bios, IRD-INERA, Burkina Faso
3Laboratoire de Biotechnologie et d’Immunologie Appliquée (LaBIA), Université Joseph Ki-Zerbo, Burkina Faso
4Laboratoire de Virologie de Biotechnologies Végétales, Ecole Supérieure d’Agronomie, Université de Lomé, Togo
5Laboratoire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmissibles par les Aliments et l’eau, LabESTA/UFR/SVT, Université Joseph Ki-Zerbo, Burkina Faso

*Corresponding Author: Fidèle Tiendrébéogo, Laboratoire de Virologie et de Biotechnologies Végétales, Institut de l’Environnement et de Recherches Agricoles (INERA), Burkina Faso.

Received: July 29, 2020; Published: August 18, 2020

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Abstract

Okra [Abelmoschus esculentus (L.) Moench] is an important crop which is widely grown in Africa. It is a major source of fiber and vitamins for diet and incomes for several women farmers. The crop is negatively impacted by okra leaf curl disease (OLCD) which is an endemic disease in Africa and is caused by a complex of begomoviruses. To determine the identity and diversity of the virus species responsible for OLCD in Togo, Okra fields were assessed and 118 okra leaves were sampled and analyzed using polymerase chain reaction (PCR) with specific primers following sequencing. During fields’ surveys, OLCD prevalence has been estimated at 64% in Dry Savannah zone, 60% in Forest zone, 53% in Littoral zone and 50% in Humid Savannah zone. Altogether, the average prevalence of the disease in Togo is about 57%. Using, PCR to diagnose the presence of begomoviruses in the samples collected, ~56% were positive. Among the begomoviruses positive samples, ~38% were found associated with betasatellite molecules. The Coat Protein gene sequencing has revealed the presence of Cotton leaf curl Gezira virus (CLCuGeV) and Okra yellow crinkle virus (OYCrV) in Togo. This study highlights the complexity of begomoviruses associated to OLCD in Togo.

Keywords: Okra; Leaf Curl Disease; PCR; Coat Protein; Begomovirus; Togo

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Citation

Citation: Fidèle Tiendrébéogo., et al. “Diversity of Begomoviruses Infecting Okra [Abelmoschus esculentus (L.) Moench] in Togo". Acta Scientific Microbiology 3.9 (2020): 29-33.



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