Mohammed Awadd Ali1*, Maged M Negm2 and Heba AEl-Asfouri3
1Masters Student, Endodontic department, Faculty of Dentistry, Cairo University, Egypt
2Professor of Endodontics, Faculty of Dentistry, Cairo University, Egypt
3Associate Professor of Endodontics, Faculty of Dentistry, Cairo University, Egypt
*Corresponding Author: Mohammed Awadd Ali, Masters Student, Faculty of Dentistry, Cairo University, Egypt
Received: December 08, 2020; Published: December 28, 2020
Aim: The aim of the present study was to compare the in vitro biological behavior and marginal adaptability of MTA, Biodentine and glass-ionomer cement as retrograde filling materials.
Methodology: The biocompatibility test was made on fibroblast cells regarding cell viability. The cells were exposed to the extracts of the materials of 1, 5 and 7 days to measure cell viability using MTT assay. Regarding marginal adaptability twenty-four single-rooted teeth were resected 3 mm from the apex. Root-end cavities were then prepared using a fissure bur and filled with one of the following materials Glass ionomer cement (GIC), Mineral trioxide aggregate (MTA) and a bioactive cement Biodentine. The roots were then sectioned longitudinally. Scanning electron microscope (SEM) was used to determine area of gaps and adaptation of the root-end filling materials with the dentin.
Results: Results showed that Biodentine and MTA maintained favorable cell viability compared with GIC over the three observation points, also Biodentine and MTA exhibited the lowest marginal gaps than GIC that showed the least adaptation with largest marginal gaps.
Conclusion: MTA and Biodentine display good cytocompatibility with normal fibroblast cells, both did not significantly affect cell proliferation and can be classified as biocompatible retrograde filing materials, While GIC significantly affect fibroblast proliferation and should be used with caution.
Concerning marginal adaptability, MTA and Biodentine showed less micro-gaps with the dentinal walls compared with GIC and they can be used successfully as retrograde filling materials.
Keywords: MTA; Biodentine; GIC; MTT; Biocompatibility; Marginal Adaptability
Citation: Mohammed Awadd Ali., et al. “A Comparative Study of Biocompatibility and Marginal Adaptability of MTA, Biodentine, and Glass Ionomer Cement as Root -End Filling Materials (Comparative In-Vitro Study)”. Acta Scientific Dental Sciences 5.1 (2021): 118-125.
Copyright: © 2021 Mohammed Awadd Ali., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.