El-Sayed Hussein Ziedan1*, Moataza Mahmoud Saad2, Mohamed Abd-El-All El-Naggar1, Khames Ahmed Hemida1, Magdy Gad-El-Rab El Samman3 and Mostafa Helmy Mostafa3
1Plant Pathology Department, National Research Centre, Cairo, Egypt
2Microbial Chemistry Department, National Research Centre, Cairo, Egypt
3Plant Pathology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
*Corresponding Author: El-Sayed Hussein Ziedan, Plant Pathology Department, National Research Centre, Cairo, Egypt.
Received: February 25, 2020; Published: April 09, 2020
Root rot of grapevine an epidemic disease causing by several soil borne fungi recorded high loss plant growth of productivity. Nine endophytic biocontrol fungal and bacterial isolates of Trichoderma spp, Bacillus spp and Pseudomonas fluorescens were tested against growth of aggressive fungal isolates causing root rot of grapevine i.e. Fusarium spp. and Botryodiplodia theobromae. Results showed that the best antagonistic endophytic isolates were Trichoderma harzianum and Bacillus subtilis. Trichoderma harzianum isolate significantly reduced mycelial linear growth and conidial sporulation of fungi tested as well as over lapping growth of fungi tested. In addition, Bacillus subtilis recorded high zone inhibition toward all pathogenic fungi tested than another bacterial isolates in this study. Some promising essential oils, organic, antioxidants acids and their combinations were tested on growth of endophytic biocontrol agents of Trichoderma harzianum and Bacillus subtilis. Results obtained indicated that sorbic acid followed by propionic acid were the least effect on mycelial linear growth and conidial sporulation of Trichoderma harzianum. In addition, sorbic acid, propionic acid (0.1%), black seed oil (4.0%) and their combination had no effect negatively on Bacillus subtilis growth. In greenhouse experiment soil drench by propionic + sorbic acids, propionic acid + sorbic acid + T. harzianum + B. subtilis were the best and significantly treatments its completely suppress (100%) root rot disease percentage and disease severity on shoots and roots of grapevine plants followed by T. harzianum as individual or combination with B. subtilis were increased morphological characters of grapevine plant i.e. length, fresh and dry weight of and root size. So, combined soil treatment by propionic acid + sorbic acid or propionic acid + sorbic acid + T. harzianum + B. subtilis as the safe fungicide alternatives for management root rot disease of grapevine.
Keywords: Bacillus subtilis; Grapevine; Root Rot; Disease; T. harzianum
Citation: El-Sayed Hussein Ziedan., et al. “Efficacy of Compatibility between Endophytic Biocontrol Agents and Abiotic Agents as Fungicides Alternative for Controlling Root Rot of Grapevine". Acta Scientific Agriculture 4.5 (2020): 10-17.
Copyright: © 2020 El-Sayed Hussein Ziedan., This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.