Acta Scientific Women's Health (ASWH)(ISSN: 2582-3205)

Research Article Volume 3 Issue 6

Amitriptyline Prescription Can Potentially Affect Pituitary-Gonadal Hormonal Axis in Female Mice

Sayyed Abbas Datlibeigi1, Amir Abdolmaleki2, Parisa Abbasi3 and Amene Nikgoftar Fathi4*

1Department of Anatomical Sciences, Medical School, Yazd University of Medical Sciences, Yazd, Iran
2Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran
3Department of Surgical Technology, Paramedic School, Hamedan University of Medical Sciences, Hamedan, Iran
4Department of Anatomical Sciences, Medical School, Arak University of Medical Sciences, Arak, Iran

*Corresponding Author: Amene Nikgoftar Fathi, Department of Anatomical Sciences, Medical School, Arak University of Medical Sciences, Arak, Iran.

Received: April 23, 2021; Published: May 26, 2021


  Amitriptyline (Ami) as a tricyclic antidepressant drug is widely used globally. Pituitary-gonadal hormonal axis with various hormones is affected by many antidepressant drugs. Thus in this experimental research, we aimed to assess the probable effects of Ami with various doses on serum levels of female sexual and gonadotropin hormones. 35 female mice (25 - 30 gr, 8 - 12 weeks old) were categorized into 5 groups (n = 7); control (received no treatments), sham (treated with 0.2 ml of distilled water), and three experimental groups (Ami 50, 75, and 100 mg/kg). Treatments were administrated orally for 4 weeks. A day following experiment, the animals were weighted and sacrificed by cervical dislocation. Thoracotomy procedure was applied and the blood was aspirated (1 ml) from right ventricle to measure blood serum levels of LH, FSH, estrogen and progesterone using ELISA assay. Animals treated with various doses of Ami represented significant (p < 0.05) decreased levels of estrogen (with the dose of 75 and 100 mg/kg Ami) and progesterone (with the doses of 100 mg/kg Ami). Also, serum levels of FSH (with the doses of 75 and 100 mg/kg Ami) showed increased trend significantly (p < 0.05). No significant (p > 0.05) differences were found in total animal body weight and serum levels of LH following Ami administration with whole doses. Ami is potentially able to reduce female sexual hormones (estrogen and progesterone) in mice with no effects on gonadotropin hormones. This fluctuation in sexual hormones probably can lead to disruption in female reproductive functions.

Keywords: Amitriptyline; Mice; Estrogen; Progesterone; FSH; LH


  1. De Zwart P., et al. "Empirical evidence for definitions of episode, remission, recovery, relapse and recurrence in depression: a systematic review". Epidemiology and Psychiatric Sciences 5 (2019): 544-562.
  2. Ferrari AJ., et al. "Burden of depressive disorders by country, sex, age, and year: findings from the global burden of disease study 2010”. PLoS Medicine11 (2013): 1001547.
  3. Yeh S., et al. "Acute macular outer retinopathy (AMOR): a reappraisal of acute macular neuroretinopathy using multimodality diagnostic testing". Archives of Ophthalmology3 (2011): 360-371.
  4. Zhou X., et al. "Systematic review of management for treatment-resistant depression in adolescents". BMC Psychiatry1 (2014): 1-9.
  5. AlAwlaqi A., et al. "Role of hormones in hypoactive sexual desire disorder and current treatment". Journal of the Turkish German Gynecological Association4 (2017): 210.
  6. Afify M., et al. "Differential effects of amitriptyline treatment on testicular and liver functions in adult male rats". New York Science Journal3 (2010).
  7. Hansen FM., et al. "The influence of sexual hormones on lipogenesis and lipolysis in rat fat cells". European Journal of Endocrinology4 (1980): 566-570.
  8. Saadia Z. "Follicle Stimulating Hormone (LH: FSH) Ratio in Polycystic Ovary Syndrome (PCOS)-Obese vs. Non-Obese Women". Medical Archives4 (2020): 289.


Citation: Amene Nikgoftar Fathi., Aet al. “Amitriptyline Prescription Can Potentially Affect Pituitary-Gonadal Hormonal Axis in Female Mice”. Acta Scientific Women's Health 3.6 (2021): 120-123.


Copyright: © 2021 Amene Nikgoftar Fathi., Aet al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Acceptance rate35%
Acceptance to publication20-30 days

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is February 15, 2023.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US