Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 4 Issue 1

New Stability Indicating RP-HPLC Method for the Quantification of Hydrochlorothiazide and Valsartan Tablets

Dhulipudi Pruthvi Krishna* and Gummadi Sowjanya

Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, India

*Corresponding Author: Dhulipudi Pruthvi Krishna, Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, India.

Received: December 26, 2019; Published: December 27, 2019

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Abstract

  A new simple isocratic reverse phase liquid chromatographic method has been developed for the quantification of Hydrochlorothiazide and Valsartan in pharmaceutical formulations (Tablets). Shimadzu Model CBM-20A/20 Alite HPLC system equipped with PDA detector with Enable C18 (150 mm × 4.6 mm i.d, 5 µm particle size) was used with a mobile phase mixture of 0.1% Acetic acid and Methanol (20:80 v/v) and flow rate of 0.8 mL/min (Detection wavelength 260 nm) for the simultaneous estimation of Hydrochlorothiazide and Valsartan. Hydrochlorothiazide and Valsartan obey Beer-Lambert’s law over the concentration range 1-30µg/ml with regression equations y = 70500x - 8392.8 (R² = 0.9992) and y = 39602x – 6449 (R² = 0.9995) respectively. The LOD and LOQ were found to be 0.3078 µg/ml and 0.9345 µg/ml for Hydrochlorothiazide and 0.3189 µg/ml and 0.9672 Valsartan respectively. Forced degradation studies were performed for the combined dosage forms and the method was validated. The method is found to be precise, accurate and robust and is suitable for the routine quality control applications in pharmaceutical formulation.

Keywords: Hydrochlorothiazide; Valsartan; RP-HPLC; Forced Degradation Studies; Validation

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Citation

Citation: Dhulipudi Pruthvi Krishna and Gummadi Sowjanya. “New Stability Indicating RP-HPLC Method for the Quantification of Hydrochlorothiazide and Valsartan Tablets". Acta Scientific Pharmaceutical Sciences 4.1 (2020): 89-94.



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