Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 4 Issue 1

Comparative Analyses of Gene Expression Profiles Following Exercise - and Electrical Stimulation - Induced Improvement of Walking Performance in Rat Claudication Model

Momoko Shiragaki-Ogitani1*, Keita Kono2, Futoshi Nara3 and Atsushi Aoyagi1

1Specialty Medicine Laboratories II, Daiichi Sankyo Co., Ltd., Tokyo, Japan
2Specialty Medicine Laboratories I, Daiichi Sankyo Co., Ltd., Tokyo, Japan
3Ube Industries. Ltd. Pharmaceuticals Research Laboratory, Japan

*Corresponding Author: Momoko Shiragaki-Ogitani, Specialty Medicine Laboratories II, Daiichi Sankyo Co., Ltd., Tokyo, Japan.

Received: December 18, 2019; Published: December 26, 2019



  Exercise is considered to be the most efficacious therapeutic approach for improving walking capacity in patients with intermittent claudication (IC). Despite its enormous benefits, the underlying mechanisms remain unclear. To reveal the local mechanisms underlying exercise-induced improvement in walking performance, we previously developed an electrical stimulation (ES) model which exerts local limb muscle contraction that mimics exercise training. This prior study showed ES-induced improvement of walking performance in a rat IC model. In the present study, we confirmed gene expression profiles following hind-limb ischemia, and recovery following exercise or ES-intervention using GeneChip microarray in a rat IC model. Of the top 40 genes upregulated or downregulated following exercise and ES compared with hind-limb ischemia, most genes changed in opposite directions to sham-operated normal control. We further confirmed the gene expression changes using semi-quantitative real-time PCR, and identified several candidates that might potentially be involved in the improvement of walking performance following exercise or ES including Dpp4, Nov, and Ptges. Further analyses of gene expression profiles and characterization of individual genes will help in the identification of therapeutic targets for the treatment of patients with IC.

Keywords: Intermittent Claudication; Exercise; Electrical Stimulation; Mechanism; Transcriptional Analysis



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Citation: Momoko Shiragaki-Ogitani., et al. “Comparative Analyses of Gene Expression Profiles Following Exercise - and Electrical Stimulation - Induced Improvement of Walking Performance in Rat Claudication Model". Acta Scientific Pharmaceutical Sciences 4.1 (2020): 67-74.

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