Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 4 Issue 2

Antihypertensive and Cardio Protective Effects of the Convolvulus arvensis root in NG-nitro-L-arginine methyl ester (L-NAME) Induced Hypertension in Swiss Albino Mice

Abida Niazi1*, Mehak Nimra2, Muhammad Bilal3, Aqsa Zarnigar3, Sadaf Nawaz4 and Fasiha Javid5

1University of Veterinary and Animal Sciences, Lahore, Pakistan
2Saulat Institute of Pharmaceutical Sciences, Quaid e Azam University, Islamabad, Pakistan
3Department of Pharmacology, Riphah Institute of Pharmaceutical Sciences, Islamabad, Pakistan
4School of Pharmacy, The University of Lahore, Islamabad, Pakistan
5Health Services Academy, Islamabad, Pakistan

*Corresponding Author: Abida Niazi, University of Veterinary and Animal Sciences, Lahore, Pakistan.

Received: January 08, 2020; Published: January 27, 2020

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Abstract

Objective: To assess antihypertensive and cardio protective effects of the Convolvulus arvensis root powder in NG-nitro-L-arginine methyl ester (L-NAME) induce hypertension in Female Swiss albino mice. 

Methodology: Female Swiss albino mice were divided into four categories. Control, 2% gum acacia L-NAME (40 mg/kg orally), CA (500 mg/kg orally) + L-NAME (40 mg/kg orally), L-arginine (100 mg/kg orally) + L-NAME (40 mg/kg orally) were given for 1 month. On 29th day serum marker enzymes, cholesterol and hemodynamic parameters (systolic and diastolic BP) were measured and cardiac histology was performed. CA root powder was characterized by HPLC. 

ResultSystolic blood pressure level was raised by L-NAME (p<0.001). In both of the drugs treated groups, systolic and diastolic blood pressure level reduced significantly (p<0.001) compared to L-NAME group. In the L-NAME group significantly (p<0.01) elevated cholesterol was reduced (p<0.05) by CA root powder treatment. In the L-NAME group, inflammation and necrosis (0-35%) were present in the heart, whereas there was no change in the myocardium of CA and L-arginine treated mice. Vitexin, Orientin and Isoorientin were identified in the Methanolic extract of CA powder. 

Conclusion: The cardio-protective activity in CA and L-arginine treated mice, was evaluated by presence of the inflammation in the heart, absence of necrosis and remarkable depletion in Serum cholesterol parameters. Inhibition of oxidation activity by Orientin and Isoorientin appears to lessen the L-NAME induced damage. It is concluded that CA root extract possess antihypertensive and cardio protective activity.

Keywords: NG-nitro-L-arginine methyl ester (L-NAME); Convolvulus arvensis; Isoorientin

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Citation

Citation: Abida Niazi., et al. “Antihypertensive and Cardio Protective Effects of the Convolvulus arvensis root in NG-nitro-L-arginine methyl ester (L-NAME) Induced Hypertension in Swiss Albino Mice" Acta Scientific Pharmaceutical Sciences 4.2 (2020): 01-08.



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