Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 4 Issue 2

The Potential of Emamectin Benzoate to Induce Kidney DNA Oxidation, Heat Shock Protein Levels and Apoptosis in Male Mice

Ozge Temiz*

Department of Biology, Faculty of Science and Letters, Cukurova University, Turkey

*Corresponding Author: Ozge Temiz, Department of Biology, Faculty of Science and Letters, Cukurova University, Turkey.

Received: January 18, 2020; Published: January 23, 2020

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Abstract

  Biopesticide Emamectin benzoate (EMB), which is used in a wide range of areas such as food production and household pest removal, causes toxic effects caused by different uses and can cause health problems in human life. As a result of pesticide exposure, it causes biological damage such as oxidative toxicity and initiation of apoptosis signal and genotoxic effects. In sublethal EMB doses; E1 (25 mg/kg/day), E2 (50 mg/kg/day), E3 (100 mg/kg/day) were administered to Swiss albino male mice for 14 days by gavage. Male mice in kidney tissues; changes in thiobarbituric acid reagent (TBARS) levels, HSP70 (heat shock proteins 70) levels known as stress protein, apoptosis marker caspase 3 enzyme activity and DNA oxidation marker 8-hydroxy-2-deoxyguanosine (8-OHdG) levels were investigated. The results of this study showed that the increase in TBARS level in kidney tissue after exposure to EMB caused lipid peroxidation. An increase in HSP70 levels were determined due to the stress response resulting from the toxicity of the tissues of the EMB groups. In addition, increases in caspase 3 enzyme activation and 8-OHdG levels were determined to cause both apoptosis and DNA oxidation. These changes in different molecular toxicology parameters indicate that the toxicity of EMB is effective in the kidneys of male mice at 14 days of exposure.

Keywords: Emamectin Benzoate; Caspase 3; HSP70; 8-OhdG; Mice; Kidney

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Citation

Citation: Ozge Temiz. “The Potential of Emamectin Benzoate to Induce Kidney DNA Oxidation, Heat Shock Protein Levels and Apoptosis in Male Mice". Acta Scientific Pharmaceutical Sciences 4.2 (2020): 01-05.



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