Chika J Mbah*
Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria
*Corresponding Author: Chika J Mbah, Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
Received: July 26, 2018; Published: August 23, 2018
Citation: Chika J Mbah. “Hyphenated Analytical Methods: Role in Pharmaceutical Analysis”. Acta Scientific Pharmaceutical Sciences 2.9 (2018).
A hyphenated technique is an on-line combination of a separation technique and one or more spectroscopic detection techniques. These techniques have received ever-increasing attention in pharmaceutical analysis over conventional chromatographic or classical spectroscopic techniques for both qualitative and quantitative analysis of unknown compounds in complex and varied matrices with a minimum of preparation.
The hyphenation involves linking liquid chromatography (LC), usually a high performance liquid chromatography (HPLC), gas chromatography (GC), or capillary electrophoresis (CE) to spectroscopic detection techniques such as photodiode array (PDA) UV-Vis absorbance, Fourier-transform infrared (FTIR), nuclear magnetic resonance (NMR), mass spectroscopy (MS), inductively coupled plasma (ICP)- mass spectroscopy. These couplings result in the introduction of any of these modern hyphenated techniques, namely GC or LC-PAD, GC or LC-FTIR, GC or LC-NMR, GC or LC-MS and CE-MS etc. Furthermore, the coupling of separation and detection techniques could entail more than one separation or detection techniques in the hyphenation process. For instance, LC-PDA-FTIR, LCPDA-NMR-MS, LC-PDA-MS, LC-MS-MS, LC-NMR-MS and GC-ICPMS and the like could be coupled.
Copyright: © 2018 Chika J Mbah. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.