Acta Scientific Pharmaceutical Sciences

Research ArticleVolume 2 Issue 7

Antibiogram of Staphylococci Isolates from Orthopaedic Patients

Obajuluwa AF1*, Olayinka BO2*, Adeshina GO2 and Onaolapo JA2

1Department of Pharmaceutics and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Kaduna State University, Kaduna, Nigeria
2Department of Pharmaceutics and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Nigeria

*Corresponding Author: Obajuluwa AF, Department of Pharmaceutics and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Kaduna State University, Kaduna, Nigeria.

Received: April 14, 2018; Published: June 05, 2018

Citation: Obajuluwa AF., et al. “Antibiogram of Staphylococci Isolates from Orthopaedic Patients”. Acta Scientific Pharmaceutical Sciences 2.7 (2018).


Background: Staphylococci has been implicated on bone infections and other infections worldwide. The increasing rate of these bacterial species to antibiotics is also alarming.

Objective: The aim of this study was to isolate Staphylococci from orthopaedic patients in a tertiary institution in Zaria, Kaduna State, Nigeria, and to compare the antibiotics susceptibility pattern of both the coagulase positive and coagulase negative Staphylococci.

Method: A total number of 104 Staphylococci isolates were collected from orthopaedic patients, biochemical tests were carried out to characterize these isolates into coagulase positive (Staphylococcus aureus) and coagulase negative Staphylococci. Disc agar diffusion method was used to determine the antibiotics susceptibility while the resistance pattern was classified as either multidrug resistance (MDR), extended drug resistance (XDR) or pandrug resistance (PDR).

Results: Out of the 104 Staphylococci isolates, 40 (38.5%) were coagulase positive, out of which 25 (62.5%) were methicillin-re-sistant Staphylococcus aureus (MRSA). Of the 64 (61.5%) coagulase negative Staphylococci, 49 (76.6%) were methicillin-resistant coagulase negative Staphylococci (MRCoNS).The coagulase positive isolates were more susceptible to antibiotics than the coagulase negative Staphylococci. Resistance pattern showed 11 (44%), 3 (12%) and 1 (4%) of the MRSA isolates were MDR, XDR, and PDR respectively while 14 (28.6%), 3 (6.1%) and 2 (4.1%) of MRCoNS were MDR, XDR, and PDR respectively.

Conclusion: High prevalence of MRSA and MRCoNS was observed and the isolates were generally resistant to beta lactam antibiotics used in this study.

Keywords: Staphylococci; Methicillin-Resistant Staphylococcus aureus; Methicillin–Resistant Coagulase Negative Staphylococci; Multi-Drug Resistance

Copyright: © 2018 Obajuluwa AF., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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