Acta Scientific Pharmacology (ASPC)

Research Article Volume 1 Issue 1

Muscarinic Receptor Mediated Spasmolytic Effects of Crude Extract and Fractions Prepared from Solenostemon rotundifolius Leaves on an Isolated Rabbit Jejunum

Harbor CI1-3, Ijeh II1, Ohaeri OC1, Ijioma SN1* and Ugwuanyi KC1

1Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
2Biotechnology Programme, National Root Crops Research Institute Umudike, Abia State, Nigeria
3Department of Zoology and Environmental Biology, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria

*Corresponding Author: Ijioma SN, Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria.

Received: December 17, 2019; Published: December 31, 2019

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  Solenostemon rotundifolius leaves have widely been used in ethnomedicine for the management of diarrhea and other diseases of the gastrointestinal tract. In this study, the spasmolytic effects of its leaf extract and fractions on normal and acetylcholine induced rhythmic contractions of an isolated rabbit jejunum were investigated to possibly validate this traditionally acclaimed anti- diarrheal property. Crude extract was prepared from the plant’s leaves and 7 fractions were obtained following fractionation of this crude extract. About 2-3 cm length of jejunum isolated from a stunned rabbit was suspended vertically in a 30 ml organ bath containing tyrode solution and bubbled with air. After equilibration, various doses of the extract, fractions and atropine were separately administered to the piece of tissue to determine their effects. Effects of graded doses of acetylcholine on the piece of tissue were also determined and repeated separately in the presence of atropine and the test extract and fractions with adequate washing preceding each administration. Results obtained showed that the effect of the crude extract on the piece of isolated tissue was inhibitory as graded doses like atropine, produced a dose dependent relaxation of the tissue, lowering the amplitude of contractions in each case with 0.33 mg/ml producing 71.42 percent inhibitory effect and 1.00 mg/ml producing an inhibitory activity of 91.67 percent. The effects of all the fractions were also inhibitory but fraction 5 produced the highest relaxation effect. Acetylcholine when applied caused dose dependent contractions which were significantly inhibited by the crude extract and fractions in a manner which compared favourably with that of atropine. The order of strengths of inhibitions of the test agents on acetylcholine induced contractions is: F5>F4>F3>CRUDE>F6>F1>F2>F7. We therefore conclude that Solenostemon rotundifolius leaf extract and fractions, having shown significant spasmolytic effects on an isolated piece of jejunum, may contain active components with anti-diarrheal potentials and be of value in the management of the disease.

Keywords:Acetylcholine; Fraction; Inhibitions; Tissue; Solenostemon rotundifolius; Spasmolytic

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Citation

Citation: Ijioma SN., et al. “Muscarinic Receptor Mediated Spasmolytic Effects of Crude Extract and Fractions Prepared from Solenostemon rotundifolius Leaves on an Isolated Rabbit Jejunum”Acta Scientific Pharmacology 1.1 (2020): 02-10.




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