Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 1

Hyperbilirubinaemia in Malaria Infected Children in Port-Harcourt

Roseanne A Okafor1*, Gloria N Wokem2, Chukwubike U Okeke3, Oluchi C Aloy-Amadi4 and Holy Brown2

1Department of Medical Laboratory Science, Pamo Medical University, Port Harcourt, Rivers State
2Department of Medical Laboratory Science Rivers State University Port Harcourt, Rivers State
3Department of Prosthetics and Orthotics, Federal University of Technology, Owerri, Imo State
4Department of Medical Laboratory Science Imo State University Owerri, Imo State

*Corresponding Author: Roseanne A Okafor, Department of Medical Laboratory Science, Pamo Medical University, Port Harcourt, Rivers State.

Received: December 02, 2019; Published: December 26, 2019

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Abstract

Background: Malaria is an endemic infection in southern region of Nigeria. This study was carried out among 1000 randomly selected school children aged 1 - 10 years to assess the effects of malaria on bilirubin metabolism.

Methods: Six hundred and ninety four (694) of the children (the test group) were of some private and government hospitals in Port Harcourt, while three hundred and six (306) apparently healthy children attending some private Nursery and Primary schools in Port Harcourt were the control group. 5ml of blood was collected from each child and dispensed into ethylene diethyl tetraacetic acid bottle and plain tubes. Whole blood in ethylene diethyl tetraacetic acid bottle was used for malaria parasite identification using thin and thick films stained with Giemsa Romanowsky stain, while the serum in plain tubes was used for bilirubin estimation using Jendrassik Groff method. The subjects were grouped into four; control group (non-infected), high malaria parasitaemic group, moderate malaria parasitaemic group and low malaria parasitaemic group.

Results: Generally, it was observed from the result that total bilirubin, conjugated bilirubin, unconjugated bilirubin, were significantly elevated (P < 0.05) in malaria infected individuals when compared to the non-infected subjects. In high malaria parasitaemic subjects, total, conjugated and unconjugated bilirubin, were elevated significantly (P < 0.05) more than that of low parasitaemic subjects, and likewise, they were significantly higher than those of moderate parasitaemic subjects. In all age groups (1 - 5, 6 - 10 and 1 - 10 years), total bilirubin, conjugated bilirubin, unconjugated were significantly (P < 0.05) elevated in malaria infected subjects when compared with non-infected subjects.

Conclusion: These significant changes in these parameters suggested that malaria parasitemia significantly causes hyperbiribinaemia among children below the age of 10 years and severity of the hyper bilirubinemia creases as the degree of parasitaemia increases. Left unchecked, this could result in bilirubin encephalopathy or child mortality.

Keywords: Children; Malaria; Bilirubin

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References

  1. Cheesbrough M. “Examination of blood for malaria parasite”. 2nd ed. United Kingdom: Cambridge University Press (1999).
  2. Cox-Singh J., et al. “Plasmodium knowlesi in humans is widely distributed and potentially life threatening”. Clinical Infectious Diseases 46.2 (2008): 165-171.
  3. Angella AF., et al. “Seasonal genetic partitioning in the geotropical malaria vector, A. darling”. Malaria Journal 13 (2014): 203.
  4. Rosenthal P. “Defining and defeating the intolerable burden of malaria. Progress and prospective”. The American Journal of Tropical Medicine and Hygiene 77 (2007): 1-327.
  5. World Health Organization. “Severe falciparum malaria. WHO communicable diseases cluster”. Transactions of the Royal Society of Tropical Medicine and Hygiene 94 (2000): 1-90.
  6. Snow RW., et al. “The global distribution of clinical episodes of P. falciparum malaria”. Nature 434.7030 (2005): 214-217.
  7. Nester EW., et al. “3rd ed. Protozoa disease”. New York: McGraw Hill Higher Education (2001).
  8. Buowari Y. “Paediatric care at general hospital, Aliero”. BMC Family Practice 9.1 (2009): 24.
  9. Ogun SA. “Management of malaria”. The Nigerian Medical Practitioner 49.5 (2006): 112-115.
  10. Abiodun R and Iyabo T. “Malaria treatment Research”. The Nigerian Medical Practitioner 49.5 (2006): 33-42.
  11. Oshikoya KA. “Malaria treatment in Lagos private clinics/Hospitals, Physicians compliance with WHO recommendations”. The Nigerian Medical Practitioner 49.5 (2006): 102-110.
  12. Federal Ministry of Health, Nigeria. “National antimalarial treatment guideline”. The Nigerian Medical Practitioner 48.1 (2005): 17-21.
  13. Baker VS., et al. “Cytokine associatied neutrophils extracellular traps and antinuclear antibodies in P. falciparum infected children under 6 years of age”. Malaria Journal 7 (2008): 41.
  14. Tanner M and Savigny D. “Malaria eradication back on the table”. Bulletin of the World Health Organization 86.2 (2008): 82. 
  15. Rijam A and Tor-Agbidye S. “The challenges of malarial infection in Nigeria”. The Nigerian Clinical Review 89.3 (2011): 6-34.
  16. Crook MA. “Liver disorders and gallstone”.7th ed. United kindom: Bookpower (2006).
  17. Baranano DE., et al. “Biliverdinereductase: a major physiologic cytoprotectant”. Proceedings of the National Academy of Sciences 99.5 (2002): 16093-16098.
  18. Viriyavejakul P., et al. “Falciparum malaria: histopathology, apoptosis and nuclear factor Kappa B expression”. Malaria Journal 13 (2014): 106.
  19. Rupani AB and Amarapukar AD. “Hepatic changes in fatal malaria; an emerging problem”. Annals of Tropical Medicine and Parasitology 103 (2009): 119-127.
  20. Cheesbrough M. “Transmission and life cycle of malaria parasite”. 2nd ed. United Kingdom: Cambridge University Press (2006).
  21. Mally HT and Evelyn KA. “The determination of bilirubin with the photoelectric colorimetric”. Journal of Biological Chemistry 112.2 (1937): 481-491.
  22. Martinek R. “Improved micro-method for determination of serum bilirubin”. Clinica Chimica Acta 13 (1996): 61-170.
  23. Warhurst DC and Willen JE. “Laboratory diagnosis of malaria”. Journal of Clinical Pathology 49 (1996): 533-538.
  24. Mishra SK., et al. “Hepatic changes in P. falciparum malaria”. Indian Journal of Malariology 29.3 (1992): 167-171.
  25. White NJ and HO M. “The pathophysiology of malaria”. The Journal of Advances in Parasitology 31 (1992): 84-167.
  26. Kausar MW., et al. “Correlation of bilirubin with liver enymes in patients of falciparum malaria”. International Journal of Surgical Pathology 8.2 (2010): 63-67.
  27. Mehta SR., et al. “Falciparum malaria. Present day problem. An experience with 44424 cases”. Journal of the Association of Physicians of India 37 (1989): 264-266.
  28. Ali H., et al. “Parasite density and the spectrum of clinical illness in falciparum malaria”. Journal of College of Physicians and Surgeons Pakistan 18.6 (2008): 362-368.
  29. Nweneka CV., et al. “Iron delocalisation in the pathogenesis of malarial anaemia”. Transactions of the Royal Society of Tropical Medicine and Hygiene 104.3 (2010): 175-184. 
  30. Kochar DK., et al. “Hepatocyte dysfunction and hepatic encephalopathy in P. falciparum malaria”. Quarterly Journal of Medicine 96.7 (2003): 505-512.
  31. Chawla LS., et al. “Jaundice in P. falciparum”. Journal of the Association of Physicians of India 43 (1989): 206-208.
  32. Ashan T., et al. “Falciparum malaria or fulminant hepatic failure”. Journal of Pakistan Medical Association 43 (1993): 206-208.
  33. Bag S., et al. “Complicated falciparum malaria”. Indian Academy of Pediatrics 31 (1994): 821-825.
  34. Satpathy SK., et al. “Severe falciparum malaria”. Indian Academy of Pediatrics 71 (2004):133-35.
  35. Bhalla A., et al. “Malaria hepatopathy”. Postgraduate Medical Journal 52.4 (2006): 315-320.
  36. Adekunle AS., et al. “Serum status of selected biochemical parameter in malaria. an animal model”. Biomedical Research 18.2 (2007): 109-113.
  37. Bhave SY., et al. “Hepatic and renal dysfunction in childhood malaria”. Bombay Hospital Journal 45 (2005): 79-84.
  38. Anand AC., et al. “Malaria hepatitis, a heterogeneous syndrome?” The National Medical Journal of India 5 (1992): 59-62.
  39. Ghoda MK. “Falciparum hepatopathy: A reversible and transient involvement of liver in falciparum malaria”. Tropical Gastroenterology 23 (2002): 70-71.
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Citation

Citation: Roseanne A Okafor., et al. “Hyperbilirubinaemia in Malaria Infected Children in Port-Harcourt". Acta Scientific Microbiology 3.1 (2020): 130-135.



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