Zinaida Klestova* and Iryna Savinova
State Scientific Control Institute of Biotechnology and Strains of Microorganisms, Kyiv, Ukraine
*Corresponding Author: Zinaida Klestova, State Scientific Control Institute of Biotechnology and Strains of Microorganisms, Kyiv, Ukraine
Received: November 25, 2019; Published: December 09, 2019
Cell lines are useful tools to facilitate in vitro studies in virology, epizootology and many other biological field of research. We describe an efficient method of primary cell culture obtaining from pool of internal parenchymal organs of tadpoles Xenopus laevis. The enzymatically digested tissue attached well to previously treated cell culture surface and start of exponential cell growth ranged from 10 to 14 days. Cells had fibroblastic morphology and normal diploid karyotype up to ten passage. African clawed frog cell culture was propagated continuously, cryopreserved and recovered successfully. Acceptable cell growth occurred at 29°C in standard medium DMEM after osmotic pressure correction and FBS adding. This cell culture would be used in the study of viral diseases of amphibian, as well as fundamental virological and epizootological investigation.
Keywords: African Clawed Frog (Xenopus laevis); Cell Culture; Amphibian; Fibroblast
Citation: Zinaida Klestova and Iryna Savinova. “Efficient Establishment of Cell Culture from Xenopus laevis as a Model for In Vitro Studies".Acta Scientific Microbiology 3.1 (2020): 42-46.
Copyright: © 2020 Lia Zinaida Klestova and Iryna Savinova. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.