Alireza Mirzaei1, MohammdAyoub Rigi Ladiz2, Fatemeh Javani-Jouni3, SeyedehSareh Hendi4, Roya Najafi-Vosough5, Hadi HashemZehi6 and Leila Gholami7*
1hD Candidate, Department of Periodontology, Bonn University, Bonn, Germanna and Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
2Oral and Dental Research Center, Department of Periodontology, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran
3Department of Microbiology, Islamic Azad University, Tehran North Branch, Postdoc Researcher Department of Biophysics, Tarbiat Modares University, Tehran, Iran
4Department of Endodontics, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran
5Department of Biostatistics and Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
6Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran
7Dental Implant Research Center, Department of Periodontology, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran
*Corresponding Author: Leila Gholami, Department of Periodontology, School of Dentistry, Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Received: November 11, 2019; Published: December 10, 2019
Purpose: Diode lasers with a 980 nm wavelengths have become popular in oral surgeries. Their cellular phototherapy effects are a topic needing further investigations. The aim of this study was to examine the effects of this wavelength on human periodontal ligament stem cells (PDLSCs) and Human gingival fibroblasts (HGF), which play an important role in periodontal tissue regeneration and oral wound healing.
Methods: HGF cell line was purchased from the Pastour National cell bank of Iran (NCBI: C-165), PDLSCs were isolated from adult human third molars and their Mesanchymal nature was confirmed by flow cytometric evaluation of CD90,CD105 and CD45 cell surface markers. Cells were irradiated with Fulences (Energy densities) of 0.5, 1.5, 2.5 J/cm2 with a 200 mw output power of a continuous wave 980 nm diode laser to determine the effect of a single session of laser therapy on the viability and proliferation of these two cell types after 24 and 48 hrs. A non irradiated group of each cell was considered as control.
Results: The PDLsc cells were positive for CD 90 and 105 and negative for CD 45. Laser radiation had a positive stimulatory effect on the viability of both cell types within 24 hrs, although only the 0.5 J/cm2 HGF group shad ignificantly higher MTT activity after 24 h ( P=0.017). This trend did not go on 48 h later and a reduction in the proliferation of both cell types compared to control groups was observed.
Conclusion: A single session of 980 nm laser application with the settings used in this study did not have a prolonged positive effect of HGF and PDLsc viability and proliferations and repeated sessions of irradiation seems to be necessary.
Keywords: Laser; Cell; Photobiomodulation Therapy; Periodontal Ligament
Citation: Leila Gholami., et al. “Effects of 980nm Diode Laser Irradiation on Gingival Fibroblasts and Periodontal Ligament Stem Cells".Acta Scientific Microbiology 3.1 (2020): 50-54.
Copyright: © 2020 Lia Leila Gholami., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.