Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 3 Issue 1

Experimentation with Animals: A Key Aspect of the 3Rs: The Genetic Quality

Alireza Mirzaei1, MohammdAyoub Rigi Ladiz2, Fatemeh Javani-Jouni3, SeyedehSareh Hendi4, Roya Najafi-Vosough5, Hadi HashemZehi6 and Leila Gholami7*

1hD Candidate, Department of Periodontology, Bonn University, Bonn, Germanna and Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
2Oral and Dental Research Center, Department of Periodontology, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran
3Department of Microbiology, Islamic Azad University, Tehran North Branch, Postdoc Researcher Department of Biophysics, Tarbiat Modares University, Tehran, Iran
4Department of Endodontics, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran
5Department of Biostatistics and Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
6Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran
7Dental Implant Research Center, Department of Periodontology, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran

*Corresponding Author: Leila Gholami, Department of Periodontology, School of Dentistry, Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

Received: November 11, 2019; Published: December 10, 2019

×

Abstract

Purpose: Diode lasers with a 980 nm wavelengths have become popular in oral surgeries. Their cellular phototherapy effects are a topic needing further investigations. The aim of this study was to examine the effects of this wavelength on human periodontal ligament stem cells (PDLSCs) and Human gingival fibroblasts (HGF), which play an important role in periodontal tissue regeneration and oral wound healing.

Methods: HGF cell line was purchased from the Pastour National cell bank of Iran (NCBI: C-165), PDLSCs were isolated from adult human third molars and their Mesanchymal nature was confirmed by flow cytometric evaluation of CD90,CD105 and CD45 cell surface markers. Cells were irradiated with Fulences (Energy densities) of 0.5, 1.5, 2.5 J/cm2 with a 200 mw output power of a continuous wave 980 nm diode laser to determine the effect of a single session of laser therapy on the viability and proliferation of these two cell types after 24 and 48 hrs. A non irradiated group of each cell was considered as control.

Results: The PDLsc cells were positive for CD 90 and 105 and negative for CD 45. Laser radiation had a positive stimulatory effect on the viability of both cell types within 24 hrs, although only the 0.5 J/cm2 HGF group shad ignificantly higher MTT activity after 24 h ( P=0.017). This trend did not go on 48 h later and a reduction in the proliferation of both cell types compared to control groups was observed.

Conclusion: A single session of 980 nm laser application with the settings used in this study did not have a prolonged positive effect of HGF and PDLsc viability and proliferations and repeated sessions of irradiation seems to be necessary.

Keywords: Laser; Cell; Photobiomodulation Therapy; Periodontal Ligament

×

References

  1. Lin F., et al. “Lasers, stem cells, and COPD”. Journal of Translational Medicine1 (2010): 16.
  2. Roelandts R. “A new light on Niels Finsen, a century after his Nobel Prize”. Photodermatology, Photoimmunology and Photomedicine3 (2005): 115-117.
  3. Ginani F., et al. “Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review”. Lasers in Medical Science8 (2015): 2189-2194.
  4. Wang Y., et al. “Photobiomodulation of human adipose-derived stem cells using 810 nm and 980 nm lasers operates via different mechanisms of action”. Biochimica et Biophysica Acta (BBA)-General Subjects 2 (2017): 441-449.
  5. Suresh S., et al. “Low-level laser therapy: A biostimulation therapy in periodontics”. SRM Journal of Research in Dental Sciences1 (2015): 53.
  6. Mitsiadis TA., et al. “Stem cell-based approaches in dentistry”. European Cells and Materials 30 (2015): 248-257.
  7. Seo BM., et al. “Investigation of multipotent postnatal stem cells from human periodontal ligament”. Lancet (London, England) 364 (2004): 149-155.
  8. Gao X and Xing D. “Molecular mechanisms of cell proliferation induced by low power laser irradiation”. Journal of Biomedical Science1 (2009): 4.
  9. Caccianiga G., et al. “Effects of laser biostimulation on the epithelial tissue for keratinized layer differentiation: an in vitro study”. Journal of Biological Regulators and Homeostatic Agents 30 (2016): 99-105.
  10. Pansani TN., et al. “Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals”. Lasers in Medical Science 32 (2017): 45-52.
  11. Crisan B., et al. “Influence of three laser wavelengths on human fibroblasts cell culture”. Lasers in Medical Science2 (2013): 457-463.
  12. Ren C., et al. “Effect of diode low-level lasers on fibroblasts derived from human periodontal tissue: a systematic review of in vitro studies”. Lasers in Medical Science7 (2016): 1493-1510.
  13. Almeida-Lopes L., et al. “Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence”. Lasers in surgery and medicine2 (2001): 179-184.
  14. Kreisler M., et al. “Low level 809-nm diode laser-induced in vitro stimulation of the proliferation of human gingival fibroblasts”. Lasers in surgery and medicine 30 (2002): 365-369.
  15. Tsai S-R and Hamblin MR. “Biological effects and medical applications of infrared radiation”. Journal of Photochemistry and Photobiology B: Biology 170 (2017): 197-207.
  16. Ravi M., et al. “The effect of biostimulation with 980-nm diode laser on postoperative pain and tissue response after surgical periodontal therapy: A pilot study”. Journal of Indian Academy of Dental Specialist Researchers 2 (2016): 47.
  17. Usumez A., et al. “Effects of laser irradiation at different wavelengths (660, 810, 980, and 1,064 nm) on mucositis in an animal model of wound healing”. Lasers in Medical Science 29 (2014): 1807-1813.
  18. Huang Y-Y., et al. “Biphasic dose response in low level light therapy”. Dose-Response 7 (2009).
×

Citation

Citation: Leila Gholami., et al. “Effects of 980nm Diode Laser Irradiation on Gingival Fibroblasts and Periodontal Ligament Stem Cells".Acta Scientific Microbiology 3.1 (2020): 50-54.



Member In






News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for March Issue
    The last date for submission of articles for regular Issues is February 29, 2020.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US