Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 5 Issue 12

Evaluation of Hemodynamics and Oxidative Stress in the Pathophysiology of L-NAME Induced Preeclampsia in Rats

Huma Quasimi1, Shazli Naaz1, Neha Dhyani2, Saumya Bhagat3, GA Khan4, Mairaj Ansari5 and Md Iqbal Alam1*

1Department of Physiology, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard, New Delhi, India

2Department of Cellular and Integrative Physiology, University of Nebraska Medical Centre, Omaha, USA

3Division of Bone and Mineral Disease, Washington University, School of Medicine, St. Louis, USA

4Department of Clinical Nutrition, College of Applied Medical Sciences, King Faisal University, Alhasa, KSA

5Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India

*Corresponding Author: Md Iqbal Alam, Professor, Department of Physiology, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard, New Delhi, India.

Received: October 25, 2022; Published: November 21, 2022


Background: Preeclampsia (PE) is a serious pregnancy disorder that accounts for 4-5% of fetal and maternal mortality and morbidity worldwide. It is imperative to understand the pathophysiology of PE to devise an effective therapy or cure for PE. In this study, we aim to characterize an animal model of PE and evaluate the hemodynamic changes and oxidative stress in PE.

Methods: Systolic and diastolic blood pressure, mean arterial pressure, and heart rate were recorded invasively along with proteinuria on the 21st day of gestation in an NG-nitro-L-arginine-methyl-ester (L-NAME) induced animal model and by phenotypic characteristics were accounted for. Alanine aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) levels were measured. Cardiac abnormalities were confirmed by electrocardiography and creatinine-kinase (CK) levels. Plasma concentrations of nitric oxide, reactive oxygen species (ROS), and antioxidants were measured.

Results: The hemodynamic parameters and proteinuria were found to be elevated in the PE group as compared to the other groups. The animal model was validated by phenotype characterization. We observed ST elevation and increased p-wave dispersion (Pd) and CK-MB levels. In PE groups, plasma levels of AST, ALT, and ALP were significantly higher. Significantly increased levels of ROS and decreased levels of antioxidants were observed. The results are significant with a p-value <0.05.

Conclusion: The L-NAME-induced animal replicates most of the clinical symptoms of PE. It may be used to study the pathophysiological aspects of preeclampsia, especially the effects on the maternal heart. Here, a rat model is used for the first time to show Pd, ST elevation, and cardiac hypertrophy.

Keywords: Preeclampsia; L-NAME; Oxidative Stress; Hemodynamics; ECG; Biochemical Tests


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Citation: Md Iqbal Alam., et al. “Evaluation of Hemodynamics and Oxidative Stress in the Pathophysiology of L-NAME Induced Preeclampsia in Rats". Acta Scientific Microbiology 5.12 (2022): 75-84.


Copyright: © 2022 Md Iqbal Alam., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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