Saranya Ganapathy and Sanjeev K Gupta*
Advanced Biotech Lab, Ipca Laboratories Ltd., Kandivli, Mumbai, India
*Corresponding Author: Sanjeev K Gupta, Advanced Biotech Lab, Ipca Laboratories Ltd., Kandivli, Mumbai, India.
Received: March 11, 2020; Published: April 14, 2020
Site-specific genetic modification has become a realistic possibility after nearly 50 years of research in the field of gene editing/ therapy. With the need for introducing exogenous fragment DNA or removing/modifying endogenous gene in/from a specific site in the genome of interest, precise genetic tools are being carefully designed for implementation. Creating Double Stranded Breaks (DSBs) at targeted sites in the genome of interest followed by endogenous cell based repair (Homology Directed Repair (HDR) or Non-Homologous End Joining (NHEJ)) results in genomic modifications . With this approach, it is possible to carry out gene insertion, augmentation, deletion, gene sequence modification and mutation correction, aiding in the development of better animal disease models, ex vivo and in vivo gene repair and engineered cell based therapies.
Keywords: Double Stranded Breaks (DSBs); Homology Directed Repair (HDR); Non-Homologous End Joining (NHEJ)
Citation: Saranya Ganapathy and Sanjeev K Gupta. “Genome Editing in Gene and Cell-Based Therapies". Acta Scientific Microbiology 3.5 (2020): 23-25.
Copyright: © 2020 Saranya Ganapathy and Sanjeev K Gupta. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.