Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 3

Bacterial Load of Clostridium Perfringens in the Aquatic Environment of Dal Lake, Kashmir

Mehak Hafeez1, Irfan Ahmad1*, Sabia Qureshi2, Zahid Kashoo2, Shaheen Farooq2, Shakeel Wani2, Oyas Asmi3, Feroz Shah4, Bilal Ahmad5, Farooz Bhat6, Sumaira Rashid1, Hussna1 and Nakeer Razak1

1Division of Fish Genetics and Biotechnology, Faculty of Fisheries, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, India
2Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, India
3Division of Fish Nutrition and Biochemistry, Faculty of Fisheries, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, India
4Division of Fish Health Management, Faculty of Fisheries, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, India
5Division of Social Sciences, Faculty of Fisheries, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, India
6Division of Fisheries Resource Management, Faculty of Fisheries, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, India

*Corresponding Author:Irfan Ahmad, Division of Fish Genetics and Biotechnology, Faculty of Fisheries, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, India.

Received: January 30, 2020; Published: February 07, 2020

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Abstract

  The study was aimed to investigate the presence of Clostridium. perfringens (C. perfringensand estimate its bacterial load in the aquatic environment of the Dal Lake, Kashmir. A total of 45 water samples (9 from each site) were collected randomly from 5 different sites viz., Dhobi Ghat, Telbal Nallah, Char-Chinari, Centaur Lake View and Dalgate of the Dal Lake. Using species-specific 16S rRNA gene amplification, all the 45 (100%) water samples were found to be positive for C. perfringens confirming the presence of C. perfringens in the lake. The overall C. perfringens load in water samples was found to be in the range of 9.2 x 10 CFU/ml to 1.87 x 102 CFU/ml, which is much less than the critical CFU level of C. perfringens (105-108). The highest average CFU/ml value was found at Dalgate (1.87 x 102), followed by Telbal Nallah (1.78 x 102), Dhobi Ghat (1.20 x 102), Centaur Lake View (1.05 x 102) and Char-Chinari (9.2 x 10), respectively. Statistically, it was observed that there is a significant difference in average CFU/ml values of C. perfringens at the five sites of the lake (P < 0.01). The current study documented the presence of C. perfringens in the aquatic environment of the Dal Lake for the first time in the Kashmir valley.

Keywords: C. perfringens; Anaerobe; 16S rRNA; PCR; CFU

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Citation

Citation: Irfan Ahmad., et al. “Bacterial Load of Clostridium Perfringens in the Aquatic Environment of Dal Lake, Kashmir". Acta Scientific Microbiology 3.3 (2020): 01-06.



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