Ana Maria Alvim Liberatore1*, Rosa Maria Silva2 and Ivan Hong Jun Koh1
1Laboratório de Pesquisa Experimental - Universidade Federal de São Paulo, Brazil
2Department of Microbiology, Parasitology and Immunology, Universidade Federal De São Paulo, Brazil
*Corresponding Author:Ana Maria Alvim Liberatore, Laboratório de Pesquisa Experimental - Universidade Federal de São Paulo, Brazil.
Received: January 11, 2020; Published: February 05, 2020
Introduction: Sepsis is a systemic infection associated with organ dysfunction. The so-called “gut hypothesis” of the Multiple Organ Dysfunction Syndrome holds that the gut may stimulate or exacerbate the host state of inflammatory response either by gut tissue hypoxia-ischemia injury or by intestinal barrier dysfunction followed by bacterial translocation (BT). BT has been associated with the worsening of the inflammatory status in experimental sepsis. In this study, we examined the kinetic changes of the aerobic and anaerobic facultative Gram-negative bacteria from gut microbiota and its relation to the BT following the Gram-positive and Gram-negative sepsis-induction in rats.
Methods: Wistar-EPM rats, weighing 200 - 250g were submitted to E. coli or S. aureus sepsis (108 CFU/mL, iv.) and, after 6, 12 and 24hs, intestinal segments, feces and mesenteric lymph nodes were removed and cultured to determine the number of aerobe and anaerobe facultative Gram-negative microorganisms. Sham group (saline injection) and Naïve animals were used as controls.
Results: The data showed the occurrence of an expressive bacterial overgrowth in small bowel since 6hs and from 12hs in the large bowel following sepsis challenge. These data suggest that the overgrowth in sepsis occurs acutely and, in a crania-caudal manner throughout the intestine. BT events also occurred acutely (6h) and at a higher rate in sepsis induced by E. coli, as compared to S. aureus (12hs) sepsis. Mortality was observed only in E. coli-sepsis. Control Sham group showed a low grade and a transient overgrowth, demonstrating that even minor surgical trauma can interfere with the gut microbiota equilibrium.
Conclusion: The significant mortality (60%) associated with the BT positivity in E.coli-sepsis points out possible participation of BT in the exacerbation of the pre-existing systemic inflammatory state in sepsis which was dependent on the sepsis-inducing bacterial strain. Furthermore, the increase of fecal bacterial overgrowth and BT event occurred concurrently suggesting that monitoring Gram-negative fecal overgrowth may predict the BT event.
Keywords: Sepsis; Bacterial Translocation; Gut Microbiota; Gut Bacterial Overgrowth; Experimental Sepsis
Citation: Ana Maria Alvim Liberatore., et al. “Sepsis-Triggering Pathogen Determines the Bacterial Translocation Profile and the Clinical Outcome". Acta Scientific Microbiology 3.3 (2020): 01-08.
Copyright: © 2020 Ana Maria Alvim Liberatore., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.