Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 3

Biodegradation of Crude Oil Through Biosurfactant Producing Bacterial Strains Isolated from Oily Sludge

Veeranna Channashettar1,2, Shaili Srivastava2* and Banwari Lal1

1Amity School of Earth and Environmental Sciences, Amity University, Gurgaon, Haryana, India
2Environmental and Industrial Biotechnology Division, The Enrgy and Resources Institute (TERI), New Delhi, India

*Corresponding Author: Shaili Srivastava, Amity School of Earth and Environmental Sciences, Amity University, Gurgaon, Haryana, India.

Received: January 24, 2020; Published: February 08, 2020

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Abstract

  The biodegradation of crude oil through biosurfactant produced by bacteria isolated from oily sludge collected from contaminated sites of three different refineries were studied. Eight bacterial isolates were screened for biosurfactant production by serial dilution technique and spread plate method. Two bacterial isolates were found capable of producing significant quantity of biosurfactant exhibited by surface tension activity (26.66 dy/cm), emulsification activity (up to 79%) and total petroleum hydrocarbon reduction (up to 92%). Gas chromatography analysis of 5 days treated MSM containing 1% crude oil as sole source of carbon and energy showed decrease in the concentration of aliphatic and aromatic hydrocarbon present in crude oil. The degradation of aliphatic and aromatic compounds of crude oil clearly indicates the production of biosurfactant by both bacterial isolates (TER-4 and TER-7).

Keywords: Biodegradation; Crude Oil; Biosurfactant; Isolate; Oily Sludge

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Citation

Citation: Shaili Srivastava.,et al.“Biodegradation of Crude Oil Through Biosurfactant Producing Bacterial Strains Isolated from Oily Sludge". Acta Scientific Microbiology 3.3 (2020): 01-06.



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