Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 3

In vitro Activity of Amorolfine, Ciclopirox, Itraconazole and Terbinafine Against Aspergillus versicolor as Agent of Onychomycosis

Viviana Ramírez-Hernández, Carolina Montero-Arias, María Isabela Vargas-Ovalle, Mariana Villalobos-Vargas, Alejandra Gómez-Arrieta, Stefany Lozada-Alvarado, Ingrid Salas-Campos and Daniela Jaikel-Víquez*

Sección De Micología Médica, Departamento De Microbiología, Facultad De Microbiología, Universidad De Costa Rica, San Pedro, Costa Rica

*Corresponding Author: Daniela Jaikel-Víquez, Sección de Micología Médica, Departamento de Microbiología, Facultad de Microbiología, Universidad de Costa Rica, San Pedro, Costa Rica.

Received: January 17, 2020; Published: February 08, 2020

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Abstract

  Cases of onychomycosis caused by non-dermatophyte filamentous fungi have increased over the years. It is worth noting that this group of fungi is resistant to fluconazole, thus the importance of determining the in vitro susceptibility patterns to the antifungals available. We determined the minimal inhibitory concentration of amorolfine, ciclopirox, itraconazole and terbinafine using the microdilution method M38-A, as described by the Clinical Laboratory and Standards Institute, of 13 isolates of Aspergillus versicolor obtained from onychomycosis. The final concentrations were: 0.13-64 μg/mL for amorolfine and terbinafine, 0.06 - 32 μg/mL for ciclopirox and 0.03-16 μg/mL for itraconazole. Also, we determined the interaction of these drugs by the checkerboard method. The MIC50 and MIC90 were ≥ 64.00 and ≥ 64.00 µg/mL for amorolfine, 4.00 and 8.00 µg/mL for ciclopirox, 1.00 and 1.80 µg/mL for itraconazole and 0.50 and 1.36 µg/mL for terbinafine, respectively. Regarding the combination of antifungals, 15.38% of the combinations of ciclopirox-itraconazole presented synergism, while the rest of the combinations showed no interaction. Thus, in vitro susceptibility testing indicates that terbinafine exhibited the highest antifungal activity and amorolfine the lowest against A. versicolor. Also, combining treatments enhances the activity of the drugs, proving a possible alternative for successful treatment of onychomycosis caused by this fungus.

Keywords: Aspergillus versicolor Amorolfine; Ciclopirox; Itraconazole; Terbinafine

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Citation

Citation: Daniela Jaikel-Víquez., et al. In vitro Activity of Amorolfine, Ciclopirox, Itraconazole and Terbinafine Against Aspergillus versicolor as Agent of Onychomycosis Acta Scientific Microbiology 3.3 (2020): 01-06.



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