Mshelia BM*, Adeshina GO and Onaolapo JA
Department of Microbiology, Faculty of Sciences, Federal University Birnin Kebbi, Kebbi State, Nigeria
*Corresponding Author: Mshelia BM, Department of Microbiology, Faculty of Sciences, Federal University Birnin Kebbi, Kebbi State, Nigeria.
Received: May 04, 2018; Published: July 27, 2018
Citation: Mshelia BM.,et al. “Comparative Study of Antibacterial Efficacy of Honey, Lemon and Standard Antibiotics against Staphylococcus aureus Isolates from Upper Respiratory Tract Infections”. Acta Scientific Microbiology 1.8 (2018).
The antibacterial effect of honey and lemon were investigated. Their use separately and in combination were compared with that of standard antibiotics against Staphylococcus aureus isolates from sputum, ear swab, nasal secretion and throat swab samples of patients from Ahmadu Bello University Teaching Hospital (ABUTH) and University Health Services (UHS) A.B.U., Zaria, Nigeria. Honey and/or lemon, and the standard antibiotics were used against the isolates using agar diffusion and broth dilution techniques in order to detect their effects. Minimum Inhibitory Concentrations (MIC) was also conducted to also check the efficacy of the agents used. Test for synergy was also carried out to know if there is synergistic, additive or antagonistic effect between the honey and the lemon. Excellent antibacterial activity was observed with lemon, honey and lemon mixture (with 50:30 to 50:10 v/v lemon/honey mixture having the best activity), Levofloxacin, Ceftriaxone and Gentamicin against the Staphylococcus aureus isolates. While less antibacterial activity was observed with Azithromycin and Amoxicillin-Clavulanic acid. There is no synergistic, nor antagonistic activity between the mixture of honey and lemon. Their activity is within the additive level (> 0.5 to ≤ 4) of Fractional Inhibitory Concentration index. This research therefore scientifically justifies the use of Honey and Lemon as an alternative medicine by the populace in the treatment of upper respiratory tract infections.
Keywords: Honey; Lemon; Standard Antibiotics; Synergistic Effect; Antibacterial Activity; Zone of Inhibition
Copyright: © 2018 Mshelia BM.,et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.