Ibrahim S1*, Danbirni S1, Abubakar UB1, Usman A1, Saidu AS2 and Abdulkadir IA1
1Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
2Department of Veterinary Public Health and Epidemiology, College of Veterianry Science, Lala Lipat Rai, University of Veterinary and Animal Science, Haryana, India
*Corresponding Author: Ibrahim S, Department of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria.
Received: March 15, 2018; Published: April 24, 2018
Citation: Ibrahim S., et al. “Estimates of Mycobacterial Infections Based on Abattoir Surveillance in Two North-Eastern States of Nigeria”. Acta Scientific Microbiology 1.5 (2018).
Tuberculosis (TB) is recognized as one of the most important threats to animals and human health causing morbidity, mortality and economic loss. It remains a major global health problem in livestock and man. In livestock, M. bovis is the most pathogenic species among the members of the Mycobacterium tuberculosis complex. It is widely distributed worldwide causing significant economic losses. In this study, we aimed at determining the prevalence of Mycobacteria in bovine carcasses in two north eastern states of Nigeria. The study was a cross-sectional study carried out at six selected abattoirs in the two states. Animals with lesions that were compatible with tuberculosis were recorded. Observations of pathological lesions of such organs as mediasternal, retrophargyngeal, submandibular, tracheobronchial lymph nodes, and, lungs, liver and intestines were also made. In Bauchi, 8,497 (9.8%) were found to be positive for gross TB lesions, while 10,505 (13.7%) were found to have TB lesions in Gombe State. Large proportion (50%) of TB lesion in both states was recorded in the respiratory pathway followed by digestive system (21%) and pre-scapular lymph nodes (18.6%). Tissue samples (300 from both states) were collected and cultured on Lowenstein Jensen media (containing either glycerol or pyruvate), 155 (51.7%) were Acid fast bacilli (AFB) positive and 82 (52.9%) were positive on culture and 15 (18.3%) were positive on Bioline® analysis. All isolates which were positive on Bioline® analyses were subjected to Genotype® MBTC analysis, (Hain assay PCR analysis) in order to differentiate them into their various species. The MBTC analysis showed, 12 (80.0%) to be Mycobacterium bovis, 2 (13.3%) Mycobacterium tuberculosis and 1 (6.6%) as unidentified using the standard banding patterns. There is also, the need to strengthen routine meat inspection and also to step up public health awareness programs on zoonotic nature of TB particularly among the abattoir workers and the herdsmen.
Keywords: Bovine Tuberculosis; Mycobacterium bovis; Mycobacterium tuberculosis; Abattoir; Hain Assay; Culture
Copyright: © 2018 Ibrahim S., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.