Sukayna M Fadlallah¹, Rima El Hajj², Jeanne El Hage², Zeina Nasser³, Nada Ghosn ³, Walid Ammar³ and Ghassan M Matar¹*

¹American University of Beirut, Beirut, Lebanon
²Lebanese Agriculture Research. Institute, Fanar, Lebanon
³Ministry of Public Health, Beirut, Lebanon

*Corresponding Author: Ghassan M Matar, Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut, Lebanon.

Received: February 09, 2018; Published: March 22, 2018

DOI: 10.31080/ASMI.2018.01.0040

Citation: Ghassan M Matar., et al. “Molecular Epidemiology and Antimicrobial Resistance of Campylobacter coli from Caeca and Carcass of Poultry in Lebanon”. Acta Scientific Microbiology 1.4 (2018).

Abstract

  Campylobacter is the primary bacterial cause of human intestinal infections worldwide. Species identification of fifty one Campylobacter positive isolates collected at two slaughterhouses in Lebanon was done using the 16 S rRNA sequencing. Antimicrobial susceptibility testing (AST) was performed using a wide range of β lactam agents and tetracycline. Polymerase chain reactions (PCR) detection of the bla_OXA-61 and tet (O) genes in resistant and susceptible isolates to ampicillin and tetracycline respectively was deter - mined. Genomic diversity of the isolates was assessed using Random Amplified Polymorphic DNA (RAPD). Sequencing analysis re - vealed that all tested isolates were Campylobacter coli . AST showed resistance in the isolates to cephalothin and aztreonam (100%), cefamandole and cefoxitin (98%), tetracycline (94%), ampicillin (49%), amoxicillin (47%), piperacillin (45%), carbenicillin (37%), ticarcillin (20%), ceftazidime (18%), cefotaxime (8%) and amoxicillin-clavulanic acid (2%). All ampicillin-resistant isolates and 84% of the ampicillin-sensitive ones carried the bla_OXA-61 gene. All tetracycline-resistant isolates were positive for the tet (O) gene with 98% encoded on plasmids. RAPD analysis revealed nine distinct clusters with a minimum percentage of 43.5% genomic similarity. This study emphasizes the importance of surveillance in identifying and controlling commonly circulating food borne pathogens.

Keywords: Campylobacter; Antimicrobial Resistance; Sequencing; Random Amplified Polymorphic DNA (RAPD); Lebanon; Poultry

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