Huang Wei Ling*
MD Infectious Disease Specialist (ID Specialist), General Practitioner, Nutrition Doctor, Acupuncturist, Pain Management, Medical Acupuncture and Pain Management Clinic, Franca, Sao Paulo, Brazil
*Corresponding Author: Huang Wei Ling, MD Infectious Disease Specialist (ID Specialist), General Practitioner, Nutrition Doctor, Acupuncturist, Pain Management, Medical Acupuncture and Pain Management Clinic, Franca, Sao Paulo, Brazil.
Received: February 06, 2018; Published: March 20, 2018
Citation: Huang Wei Ling. “Why do Patients Still Catch Hospital Infections Despite the Practice of Infection Prevention and Control Programs?”. Acta Scientific Microbiology 1.4 (2018).
Aeromonas hydrophila is a Gram-negative motile bacillus responsible for substantial losses in global aquaculture, which also poses a risk to human health. Aeromonosis outbreaks are associated with excess organic matter in water supplies, failure in management, primary parasitic infestations, and the presence of powerful bacterial virulence factors. This study validated an adhesin gene from A. hydrophila ( ahaI ) using a quantitative real-time PCR assay based on SYBR Green I. The analytical specificity and sensitivity of the test was analyzed. The ability to identify and quantify A. hydrophila samples isolated using classical microbiology techniques and total DNA extracted directly from Nile tilapia, Oreochromis niloticus , kidney tissue experimentally infected with bacteria was also deter- mined. The assay had 100% specificity and the sensitivity was 1.9 log gene copies of detection for total DNA from tissue. This study constitutes a valuable tool for the rapid and accurate diagnosis of A. hydrophila in intensive fish farming scenarios.
Keywords: Hospital Infection; Prevention; Control Programs; Energy; Traditional Chinese Medicine; Acupuncture; Galen; Hippocrates
Copyright: © 2018 Huang Wei Ling. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.