Manisha Mandal¹, Biswajit Ghosh², Debashis Halder², Jayashree Konar³, Nishith Kumar Pal³ and Shyamapada Mandal²*

¹Department of Physiology, MGM Medical College and LSK Hospital, Kishanganj, Bihar, India
²Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of Gour Banga, Malda, India
³Department of Microbiology, NRS Medical College and Hospital, Kolkata, India

*Corresponding Author: Shyamapada Mandal, Professor, Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of Gour Banga, Malda, West Bengal, India. E-mail: samtropmed@gmail.com

Received: January 19, 2018; Published: February 19, 2018

DOI: 10.31080/ASMI.2018.01.0027

Citation: Shyamapada Mandal., et al. “Diagnosis of Dengue Infection Cases by serum Lactate Dehydrogenase Profiling Compared to Dengue NS1 Antigen and Anti-dengue IgM Antibody Detection: A Retrospective Study”. Acta Scientific Microbiology 1.3 (2018).

Abstract

  Dengue is an acute infection with potential fatal complications; the specific antibody detection for which is an indirect method, and the novel parameter NS1 is reliable for diagnosis of dengue infection, while the serum LDH has been reported to be elevated in severe dengue. This study attempts to evaluate the association of serum LDH with NS1 antigen and IgM in dengue. The serum NS1 and IgM of dengue cases, by ELISA, were estimated from index values by dividing absorbance at 450 nm with cut-off values. Total LDH was measured using autoanalyzer. Electropherotyping of LDH was done in 7.5% polyacrylamide gels. The NS1 and IgM param - eters were compared with LDH levels, and analyzed statistically. The NS1 antigens were 35.23 ± 5.37 U (normal < 9 U) with 634.67 ± 350.55 U/L LDH (normal ≤250 U/L) (r = -0.57) among 32.33 ± 7.13 years patients and IgM antibodies were 52.38 ± 7.68 U with 451.67 ± 119.74 U/L LDH (r = -0.13) among 25.92 ± 5.01 years patients. The electropherotype showed intense and greater band pattern when IgM was detected compared to NS1 antigen. Appearance of dengue NS1 antigen as well as anti-dengue IgM in the early phase of infection was inversely related to the level of serum LDH, and increased LDH activity was found when IgM was detected. Thus, LDH might be useful as predictive tool in the early course of dengue infection.

Keywords: Dengue; NS1 Antigen; Anti-DENV IgM Antibody; ELISA; Serum Lactate Dehydrogenase; PAGE Analysis

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