Acta Scientific Microbiology

Research Article Volume 1 Issue 3

Identification, Antagonistic Potentials and Plasmid Profiling of Micro-Organisms Associated with Termitarium from Cocoa Trees in Ibule-Soro, Akure Nigeria

JO Aribisala*, MK Oladunmoye, OI Afolami and T Aina

Department of Microbiology, Federal University of Technology, Akure, Nigeria

*Corresponding Author: JO Aribisala, Department of Microbiology, Federal University of Technology, Akure, Nigeria.

Received: January 02, 2018; Published: February 03, 2018

DOI: 10.31080/ASMI.2018.01.0020

Citation: Afolami OI., et al. “Characterization, Antibiotic Sensitivity Patterns, Plasmid Profile Analysis and Antagonistic Potentials of Microorganisms from Termitaria on Mango Trees in Ibule-Soro, Akure, Nigeria””. Acta Scientific Microbiology 1.3 (2018).


Abstract

  This research identified micro-organisms associated with termitarium on cocoa trees and cocoa tree bark cultivated in Ibule-soro, Akure, Ondo State Nigeria. Bacteria such as Kocuria spp, Micrococcus spp, Bacillus cereus and Clostridium botulinum were identified, while fungi isolates such as Histoplasma capsulatum, Penicillium notatum, Microsporum audouinii, Aspergillus flavus, Cladosporium cladosporioides were also identified from the termitarium sampled from the farm settlements in Ibule-soro in Ondo State Nigeria. The antagonistic properties of the isolated bacteria and fungi were evaluated to determine the growth inhibitory effects of the isolated organisms against some selected pathogens. The selected pathogens were Escherichia coli and Shigella spp for the gram negative and Staphylococcus aureus for the gram positive. Antimicrobial sensitivity patterns of isolated bacteria from termitarium and cocoa tree bark were also evaluated using Kirby-Bauer test. The multiple drug resistant isolates (MDRIs) which include Kocuria spp and Micro- coccus spp were screened out of the bacteria isolates obtained. Plasmid profile analysis of screened multiple drug resistant isolates revealed possession of plasmids with Kocuria spp and Micrococcus spp having a plasmid weight of 1000 bp and 980 bp respectively. Plasmid curing of the selected multiple drug resistance isolates was carried out and the cured bacterial isolates were subjected again to broad spectrum antibiotic test to determine the basis of the antibiotic resistance. The initially observed antibiotic resistance for MDRIs was extra chromosomal since screened isolates were susceptible to broad spectrum antibiotics after curing. The Antagonistic test shows that only Penicillium notatum had mild antagonistic effect on the selected test pathogens while the remaining fungi and bacterial isolate had no significant antagonistic properties.

Keywords: Antagonistic; Plasmid Profiling; Termitarium; Resistance; Cocoa Trees

Bibliography

  1. Holt J and Leepage M. “Termites and soil properties: Evolu- tion, sociology, symbiosis, ecology”. Kluwer Academic Publication, Amsterdam, the Netherland (2000): 234-244.
  2. Longair R. “Tusked males, male dimorphism and nesting be- haviour in a sub-social Afro tropical wasp, Synagris cornuta and weapons and dimorphism in the genus (Hymenophera, vespidea, enumenie)”. Journal of the Kansas Entromological Society 77.4 (2004): 528-557.
  3. Cranshaw W., et al. “Bugs Rule: An Introduction to the World of Insects”. Princeton University Press, New Jersey (2013): 188-190.
  4. Culliney T and Grace J., “Prospects for the biological control of subterranean termites (Isoptera: Rhinotermitidae), with special reference to Coptotermes formosanus”. Bulletin of En- tomological Research 90 (2000): 9-21.
  5. Divina F and Divina M. “Foods of the Americas”. Washington, D.C.: National Museum of the American Indian in association with Ten Speed Press (2004).
  6. Dahlquist R M., et al. “Incorporating Livelihoods in Biodiver - sity Conservation: A Case Study of Cocoa Agroforestry Sys- tems in Talamanca, Costa Rica”. Biodiversity and Conservation, Springer Science 16.8 (2007): 2311-2333.
  7. Fall S., et al. “Differences between bacterial communities in the gut of a soil-feeding termite (Cubitermes nio- koloensis) and its mounds”. Applied and Environmental Microbiology 73.16 (2007): 5199-5208.
  8. Fawole M O and Oso B A. “Laboratory manual on microbiol- ogy”. Spectrum books limited, Ibadan, Nigeria (2007): 127.
  9. Sarah K., et al . “Descriptions of Medical Fungi”. Department of Molecular and Cellular Biology, School of Biological Sciences, University of Adelaide, Australia. 3 rd edition (2016).
  10. Cheesebrough M. “District laboratory practice in tropical countries”. Part 2. Low price edition. Cambridge University Press, London (2006).
  11. Fokkema N J and Heuvel J V. “Microbiology of the Phyllo - sphere”. Cambridge [Cambridge shire] New York: Cambridge University Press (1996): 392.
  12. Clinical and Laboratory Standards Institute (CLSI). “Perfor - mance standards for antimicrobial disk and dilution sus- ceptibility tests for bacteria isolated from animals Approved standard Third edition, CLSI document M31-A3, Clinical and Laboratory Standards Institute, 940 West Valley Road, Wayne Pennsylvania, USA, 28 (2008): 1-99.
  13. Brown T. “Vectors for Gene Cloning: Plasmids and Bacterio - phages. Gene Cloning and DNA Analysis: An Introduction”. Academic Press U.S.A (2010): 64-76.
  14. Nicoletti R., et al . “Bioprospecting for antagonistic Penicillium strains as a resource of new antitumor compounds”. World Journal of Microbiology 24.2 (2009): 185-195.
  15. Adeigbe O., et al. “Review on cashew research and production in Nigeria in the last four decades”. Scientific Research and Es - says 10.5 (2015): 196-209.

Copyright: © 2018 JO Aribisala., et al This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



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