Characterization, Antibiotic Sensitivity Patterns, Plasmid Profile Analysis and Antagonistic
Potentials of Microorganisms from Termitaria on Mango Trees in Ibule-Soro, Akure, Nigeria
Afolami OI*, Aribisala JO, Oladunmoye MK, Wasiu OS and Arogundade IO
Department of Microbiology, Federal University of Technology, Akure, Ondo State, Nigeria
*Corresponding Author: Afolami OI, Department of Microbiology, Federal University of Technology, Akure, Ondo State, Nigeria.
Received: December 20, 2017; Published: February 01, 2018
Over the years it has been proven that a mutualistic relationship exists between termites and microorganisms inhabiting their gut.
Microbiological analyses were carried out on termitarium, macerated dead termites and mango barks from farm settlements in Ibule
– soro Akure, Ondo state, Nigeria. Bacteria isolates such as
Pseudomonas aeruginosa, Clostridium botulinum, Bacillus subtilis, Actino-
sp were identified while fungal isolates such as
Chrysosporium tropicum, Mortierella rolfi, Aspergillus niger,
were also identified from the analyzed samples. Isolates such as
Aspergillus niger, Mortierella rofli
showed mild antagonism against selected clinical pathogens used; the
selected pathogens include:
Staphylococcus aureus, Salmonella
spp and Streptococcus pyogenes respectively. The results obtained
from antibiotic sensitivity tests showed that Microbacter sp and
were multiple antibiotic resistant isolates
(MDRIs). However, molecular characterization of these multiple antibiotic resistant isolates was carried out via plasmid profiling to
determine the size of the bacterial plasmids. These isolates were again subjected to antibiotics treatment after being cured of their
plasmids to determine if their initial resistance to antibiotics is chromosomal or extra – chromosomal. The findings of this research
revealed that termite nests serve as reservoirs for microbes which possess antagonistic properties and that the genetic basis for
antibiotic resistance of screened multiple antibiotic resistant bacteria isolated from termite nests were plasmid based. It is therefore
recommended that advanced culture independent approaches should be used to explore the microbial diversity of the termite nests
in the near future for greater research focus.
Keywords: Termitarium; Antibiotic Resistance Patterns; Antagonistic Patterns; Plasmid Profile Analysis; Mango Tree
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