Acta Scientific Microbiology

Research Article Volume 1 Issue 3

Characterization, Antibiotic Sensitivity Patterns, Plasmid Profile Analysis and Antagonistic Potentials of Microorganisms from Termitaria on Mango Trees in Ibule-Soro, Akure, Nigeria

Afolami OI*, Aribisala JO, Oladunmoye MK, Wasiu OS and Arogundade IO

Department of Microbiology, Federal University of Technology, Akure, Ondo State, Nigeria

*Corresponding Author: Afolami OI, Department of Microbiology, Federal University of Technology, Akure, Ondo State, Nigeria.

Received: December 20, 2017; Published: February 01, 2018

DOI: 10.31080/ASMI.2018.01.0019

Citation: Afolami OI., et al. “Characterization, Antibiotic Sensitivity Patterns, Plasmid Profile Analysis and Antagonistic Potentials of Microorganisms from Termitaria on Mango Trees in Ibule-Soro, Akure, Nigeria””. Acta Scientific Microbiology 1.3 (2018).


Abstract

  Over the years it has been proven that a mutualistic relationship exists between termites and microorganisms inhabiting their gut. Microbiological analyses were carried out on termitarium, macerated dead termites and mango barks from farm settlements in Ibule – soro Akure, Ondo state, Nigeria. Bacteria isolates such as Pseudomonas aeruginosa, Clostridium botulinum, Bacillus subtilis, Actino- bacter sp and Microbacter sp were identified while fungal isolates such as Chrysosporium tropicum, Mortierella rolfi, Aspergillus niger, Fusarium oxysporum and Penicillium chrysogenum were also identified from the analyzed samples. Isolates such as Actinobacter sp, Aspergillus niger, Mortierella rofli and Penicillium chrysogenum showed mild antagonism against selected clinical pathogens used; the selected pathogens include: Staphylococcus aureus, Salmonella spp and Streptococcus pyogenes respectively. The results obtained from antibiotic sensitivity tests showed that Microbacter sp and Pseudomonas aeruginosa were multiple antibiotic resistant isolates (MDRIs). However, molecular characterization of these multiple antibiotic resistant isolates was carried out via plasmid profiling to determine the size of the bacterial plasmids. These isolates were again subjected to antibiotics treatment after being cured of their plasmids to determine if their initial resistance to antibiotics is chromosomal or extra – chromosomal. The findings of this research revealed that termite nests serve as reservoirs for microbes which possess antagonistic properties and that the genetic basis for antibiotic resistance of screened multiple antibiotic resistant bacteria isolated from termite nests were plasmid based. It is therefore recommended that advanced culture independent approaches should be used to explore the microbial diversity of the termite nests in the near future for greater research focus.

Keywords: Termitarium; Antibiotic Resistance Patterns; Antagonistic Patterns; Plasmid Profile Analysis; Mango Tree

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Copyright: © 2018 Afolami OI., et al This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



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