Copro- Diagnosis of Early
Infection in Experimental Animals
Dalia S Ashour*, Ibrahim A Aboul Assad, Samy I El-Kowrany and Amira E Abdel Ghaffar
Department of Medical Parasitology, Faculty of Medicine, Tanta University, Tanta, Egypt
*Corresponding Author: Dalia S Ashour, Associate Professor, Department of Medical Parasitology, Faculty of Medicine, Tanta University,
December 11, 2017; Published: January 30, 2018
The early phase of trichinellosis is difficult to diagnose due to the non-specificity of the signs and symptoms that may be mis
diagnosed as alimentary intoxication or enterobacterial infection. Therefore, it is important to study early diagnostic methods for
infection to allow medical intervention at early stages of the disease. Coproantigen has become an important
alternative method for the diagnosis of intestinal infections caused by either protozoa or helminths. To investigate the sensitivity of
coproantigen detection in comparison with other traditional serodiagnostics, the following techniques were performed on
infected rats: examination of the stool for
adult or newborn larvae, detection of coproantigen by capture ELISA and PCR,
ELISA for detection of
antibodies in serum and PCR for detection of
DNA in the serum. We found that
coproantigen by ELISA and PCR show positivity from 1st
day until 9th
day post infection (P.I.). PCR detected
DNA in serum
antibodies in sera detected by ELISA technique show positivity starting from 10th
day P.I. till the end of the
adults or larvae were detected in stool. In conclusion, early diagnosis of
showed higher sensitiv
ity by copro-PCR and copro-ELISA followed by detection of
DNA in serum then detection of
antibodies in serum.
Keywords: Trichinella spiralis; Coproantigen; Copro-ELISA; Copro-PCR
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