Evaluation of Antimicrobial Efficacy of Punica Granatum, Green Tea, Chitosan
and Sodium Hypochlorite Irrigants Against E. faecalis
Sadia Ada*, Merlin Ann Joseph, Jayalakshmi KB, Shibani Shetty,
Sujatha I, Prasanna Latha Nadig and Manje Gowda
Department of Conservative Dentistry and Endodontics, Krishnadevaraya College of Dental Sciences and Hospital, India
*Corresponding Author: Sadia Ada, Department of Conservative Dentistry and Endodontics, Krishnadevaraya College of Dental Sciences and Hospital, India.
Received: March 14, 2022; Published: April 07, 2022
Aim: This study aimed to compare the antimicrobial efficacy of various irrigants- Punica granatum 20%, green tea extract 3%, chitosan 2% and sodium hypochlorite 3% against E. faecalis
Materials and Methodology: E. faecalis strain ATCC 29212 colonies were collected from cultures grown on brain–heart infusion agar. Extracts of various test irrigants- Punica granatum 20%, green tea 3% and chitosan 2% were prepared. Wells of 6 mm diameter were cut in the prepared agar medium using sterile templates. 50 μL of the test reagents were inoculated into the wells under aseptic conditions. These plates were then incubated at 37 ̊ C for 24 hours. The zone of inhibition of growth around the wells was then measured. Data were analyzed using one-way analysis of variance and Tukey's post hoc test. Descriptive statistics was obtained using SPSS software (version 11.5) with P established at < 0.05
Results: The results indicated that Punica granatum showed the largest zone of inhibition with a mean value of 24mm followed by Sodium hypochlorite at 18.8mm, green tea at 13.8mm and chitosan extract showed a zone of 11.7mm respectively.
Conclusion: Punica granatum 20% had the greatest potential to be used as an endodontic irrigant against E. faecalis among the tested irrigants.
Keywords:Punica Granatum; Chitosan; Green Tea; Sodium Hypochlorite; E. faecalis
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