Acta Scientific Dental Sciences

Research ArticleVolume 2 Issue 6

Determination of Mesenchymal Stem Cell Origin during Bleeding-Induced Regenerative Endodontic Procedure Using 2-Step Real-Time Reverse-Transcription Polymerase Chain Reaction (qRT-PCR)

Mostafa I Negm1, Jealan M ElShafei1, Nagwa K Roshdy2* and Ghada E Eid1

1Departments of Endodontics, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt
2Medical Biochemistry and Molecular Biology, Future University, Cairo, Egypt

*Corresponding Author: Nagwa K Roshdy, Medical Biochemistry and Molecular Biology, Future University, Cairo, Egypt.

Received: March 12, 2018; Published: May 07, 2018

Citation: Nagwa K Roshdy., et al. "Determination of Mesenchymal Stem Cell Origin during Bleeding-Induced Regenerative Endodontic Procedure Using 2-Step Real-Time Reverse-Transcription Polymerase Chain Reaction (qRT-PCR)”. Acta Scientific Dental Sciences 2.6 (2018).

Abstract

Introduction: This study evaluated possible stem cells origin during regenerative endodontic procedure, whether from surviving dental pulp stem cells within disinfected root canals walls or from stem cells of apical tissues or they act synergistically.

Methods: Immature necrotic permanent single rooted-teeth (n = 30) of patients (n = 24) 7-18 years old were divided into two groups according to presence or absence of apical radiolucency: group A with apical radiolucency and group B with healthy periodontium. After informed consent, two-sessions regenerative endodontic protocol was implemented. First session root canals were disinfected using 1.5% NaOCl irrigate and Bimix medicament for 3 weeks. Second session root canals were irrigated using 17% EDTA followed by a final saline rinse. Saline samples were collected from disinfected root canals using sterile paper points. Periodontium was apically stimulated using hand files until bleeding reached the cementoenamel junction for the assumed stem cells delivery. Blood samples were collected from root canals using sterile paper points. The expression of specific mesenchymal stem cell gene markers; CD105 and CD73 was assessed using 2-step qRT-PCR relative to infected root canals. Mann-Whitney U test was used for comparison. Statistical significance was set at “p ≤ 0.05”.

Results: In group A, the fold increase for CD105 and CD73 in saline samples were 19.6% and 7.6%, respectively, while the fold increase in blood samples were 26.6% and 17.8% with statistically significant difference, P-value < 0.001. Same trend was observed in group B, the fold increase for CD105 and CD73 in saline samples were 20.2% and 8%, respectively, while the fold increase in blood samples were 26.2% and 17.6% with statistically significant difference, P-value < 0.001.

Keywords: Mesenchymal; Regenerative Endodontics; qRT-PCR, Immature; Necrotic

Copyright: © 2018 Nagwa K Roshdy., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



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