Sreejith VP1, Shermil Sayd2*, Sameena Shamsudeen3 and Mohamed Jamsher3
1Professor and Head of the Department, Department of Oral and Maxillofacial Surgery Kannur Dental College, Kannur, India
2Assistant Professor, Department of Oral and Maxillofacial Surgery, Kannur Dental College, Kannur, India
1Postgraduate trainee, Department of Oral and Maxillofacial Surgery, Kannur Dental College, Kannur, India
*Corresponding Author: Shermil Sayd, Assistant Professor, Department of Oral and Maxillofacial Surgery, Kannur Dental College, Kannur, India.
Received: February 28, 2018; Published: April 05, 2018
Citation: Shermil Sayd., et al. “Identification of Microbial Isolations from the Clinical Aprons of Graduates and Post-Graduate Trainees in a College Department”. Acta Scientific Dental Sciences 2.5 (2018).
Aim of the Study: To determine the microorganisms present on the white aprons of the students, interns and post-graduates in the Department of Oral and Maxillofacial Surgery outpatient department.
Settings and Design: A survey of the random sample consisted of 18 white aprons of dental students, interns and postgraduate students were done over a period of one week who agreed to participate in the study.
Methods and Material: Sample consisted of 18 white aprons of dental students, interns and postgraduates, three most representative locations of each apron were analyzed, over a period of six working days, who agreed to participate in the study. For each participant, three sites of their aprons were selected for microbial collection by the way of using a sterile cotton swab: sleeve, pocket and lapel. Normal saline was used to moisten the swabs before collecting the sample by passing the swabs up and down, twice on the desired areas and they were immediately sent for microbial culture. The swabs that were received by the department of microbiology were immediately streaked onto blood agar and McConkey agar and the plates were incubated overnight at 370C. The colonies obtained were identified by using standard techniques. Antibiotic sensitivity testing was done by Mueller-Hinton agar.
Results: Out of the 18 participated, 48% (95% CI = 34.8% to 61.4%) were contaminated with Staphylococcus (staph.) aureus and from that, 18.5% [95% confidence interval (CI) = 8.1% to 28.9%] were contaminated with Methicillin Resistant Staph. aureus (MRSA), 7 identified on the lapel (26.9%), 11 identified on the pocket (42.3%) and 8 identified on the sleeve (30.8%).
Keywords: White Apron; Bacterial Contamination; Nosocomial Infection; MRSA
Copyright: © 2018 Shermil Sayd., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.