Acta Scientific Dental Sciences

Research ArticleVolume 2 Issue 4

An Assessment of the Neurotoxicity of Different Root Canal Sealers on Rat Sciatic Nerve: An Electrophysiologic and Histopathologic Study

Ahmed M El Gendi1*, Jealan M El Shafei1, Heba A El Asfouri1 and Mohamed A Eshra2

1Department of Endodontics, Faculty of Oral and Dental Medicine, Cairo University, Egypt
2Department of Medical Physiology, Faculty of Medicine, Cairo University, Egypt

*Corresponding Author: Ahmed M El Gendi, Department of Endodontics, Faculty of Oral and Dental Medicine, Cairo University, Egypt.

Received: February 26, 2018; Published: March 20, 2018

Citation: Ahmed M El Gendi., et al. “An Assessment of the Neurotoxicity of Different Root Canal Sealers on Rat Sciatic Nerve: An Electrophysiologic and Histopathologic Study”. Acta Scientific Dental Sciences 2.4 (2018).

Abstract

Objective: The objective of this study was to compare the neurotoxic effect of Endosequence BC sealer and MTA FilLapex sealer versus AH-plus by assessing the nerve conduction velocity (NCV) of isolated rat sciatic nerve and features of histopathological neurotoxic effect.

Methods: Fifty seven white female albino rats were used in this study and grouped into three equal groups, 19 rats for each sealer group. All sealers were prepared according to manufacturer’s instruction and applied on the all entire length of the isolated rat sciatic nerve. The neurotoxicity of the root canal sealers (RCSs) was assessed by calculating the (NCV) of isolated rat sciatic nerve before, after 5 minutes and after 30 mins of sealer application on the nerve by the electrophysiologic data acquisition system (power lab) device. NCV can determine the nerve destruction and damage. Sciatic nerve samples that revealed a significant decrease in NCV due to severe neurotoxic effects had been selected for subsequent histopathological analysis.

Results: The only statistical significant difference of the NCVs between groups was observed after 30 minutes between AH-Plus and Endosequence and between AH-Plus and MTA Fillapex so AH-Plus has the most neurotoxic effects followed by MTA Fillapex and the least one was Endosequence bc Histological examination of haematoxylin and eosin (H&E) stained sections of sciatic nerves with a significant decrease in NCV revealed that there were marked histopathological changes for AH-plus group compared to Endosequence BC and MTA Fillapex groups. Due to the very limited research papers on the neurotoxicity of the endodontic materials we conducted our study. Conclusion: Overextension with pushing of resin based cement into periapical tissue should be avoided other wise a paraesthesia might occurs moreover, measuring the NCV was proved to be a valuable tool in assessing the neurotoxic effect of the root canal sealer and Histopathological data are essential tool to confirm the electrophysiological findings.

Conclusion: Overextension with pushing of resin based cement into periapical tissue should be avoided otherwise a paraesthesia might occurs moreover, measuring the NCV was proved to be a valuable tool in assessing the neurotoxic effect of the root canal sealer and Histopathological data are essential tool to confirm the electrophysiological findings.

Clinical Significance: Apical extrusion of endodontic filling materials may cause Inflammation, nerve toxicity and damage so; extrusion of root canal filling should be avoided during endodontic treatment.

Keywords: Neurotoxicity; Root Canal Sealer; AH-Plus; Endosequence BC; MTA Fillapex; Rat Sciatic Nerve

Copyright: © 2018 Ahmed M El Gendi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



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