Bhaskaran Sathyapriya^1,2*, KR Srinivasan³and Purushothaman Lakshmanan⁴

¹Research Scholar, Department of Anatomy, Vinayaka Missions Aarupadai Veedu Medical College and Hospital, Vinayaka Missions University, Salem, Tamilnadu, India
²Reader, Department of Anatomy, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Chennai, Tamilnadu, India
³Professor, Department of Anatomy, Vinayaka Missions Aarupadai Veedu Medical College and Hospital, Vinayaka Missions University, Salem, Tamilnadu, India
⁴Consultant Orthodontist, Apollo Hospitals and Apollo White Dental, Chennai, Tamilnadu, India

*Corresponding Author: Bhaskaran Sathyapriya, Research Scholar, Department of Anatomy, Vinayaka Missions Aarupadai Veedu Medical College and Hospital, Vinayaka Missions University and Reader, Department of Anatomy, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Tamilnadu, India.

Received: June 12, 2018; Published: June 28, 2018

Citation: Bhaskaran Sathyapriya., et al. “Evaluation of Micronuclei Frequency in Peripheral Blood Lymphocytes of Patients with Oral Squamous Cell Carcinoma". Acta Scientific Cancer Biology 2.5 (2018).

Abstract

Aim: To assess DNA/chromosome damage in peripheral blood lymphocytes (PBL) of patients with oral carcinoma by micronucleus assay.

Materials and Methods: DNA damage was assessed in PBL of 10 oral cancer patients using micronucleus assay. Samples of venous blood (3 ml) were collected in heparinized vials under sterile conditions on day 1 of the first radiotherapy cycle 2 hours before irradiation (sample 1), 2 hours after the application of the first dose (sample 2), during the middle of the radiotherapy (sample 3) and after 6 months (sample 4) after radiotherapy.

Results: The frequency of structural chromosome aberrations in lymphocytes gradually increased during the radiation cycle. In pretherapy sample 1, the mean total number of micronuclei of 23.00 ± 3.559. After administration of the first fraction of radiotherapy (sample 2), the mean total number of micronuclei was 29.60 ± 3.239. In most participants, the highest level micronuclei frequency was recorded in blood samples collected during the middle of the radiotherapy (138.50 ± 20.22). Later on, the levels of primary DNA damage slightly diminished (53.30 ± 13.881).

Conclusions: In conclusion, introduction of genotoxicological methods in medical surveillance of cancer patients before and after radiotherapy could be important in evaluating secondary cancer risk and, in case of cancers such as oral carcinoma which may have initial genome burden, could be an important factor for individual therapy adjustment.

Keywords: Micronuclei; Lymphocytes; Radiotherapy; Oral Carcinoma; Chromosome Damage

Copyright: © 2018 Bhaskaran Sathyapriya., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.