Acta Scientific Biotechnology (ASBT)

Research Article Volume 1 Issue 1

IgY: A Promising Alternative to IgG in Immunodiagnostics

Kota Rohini Krishna, Krupanidhi Sreerama and Prakash Narayana Reddy*

Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research (Deemed to be University), Vadlamudi, Andhra Pradesh, India

*Corresponding Author: Prakash Narayana Reddy, Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research (Deemed to be University), Vadlamudi, Andhra Pradesh, India.

Received: December 11, 2019; Published: December 31, 2019

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Abstract

  IgY is a predominant antibody found in reptiles, birds and amphibians. IgY is equivalent to IgG of mammals. In the present study, IgY antibodies against Shigella flexneri was produced by immunizing chicken with 0.2 ml of 3.6x109 CFU ml-1 of formalin inactivated Shigella flexneri ATCC 9199 strain. Eggs were collected and IgY antibodies were harvested using PEG precipitation method. Yield of ~25 mg of IgY per egg yolk was recovered. Dot ELISA with immunized IgY showed reactivity with Shigella flexneri and also other Shigella species, while naïve IgY did not exhibit any reactivity. IgY cross-reactivity with other Shigella members indicate the prosect of using IgY in detection and neutralization of pathogenic Shigella strains.

Keywords: Immunoglobulin Y; Egg Yolk; Shigella flexneri; Dot ELISA; UV Spectrum

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References

  1. Kota Rohini Krishna., et al. “IgY antibodies of chicken do not bind staphylococcal binder of immunoglobulin (Sbi) from Staphylococcus aureus”. Annals of Microbiology 69.5 (2019): 531-540.
  2. Jenkins JR. “Rodent diagnostic testing”. Journal of Exotic Pet Medicine 17 (2008): 16-25.
  3. Lee Warren., et al. “Insights into the chicken IgY with emphasis on the generation and applications of chicken recombinant monoclonal antibodies”. Journal of Immunological Methods 447 (2017): 71-85.
  4. Michael Nancy M., et al. “Methods for the production of chicken monoclonal antibodies”. U.S. Patent No. 6,143,559. (2000).
  5. Saylor Carolyn., et al. “Monoclonal antibody-based therapies for microbial diseases”. Vaccine 27 (2009): G38-G46.
  6. Kovacs-Nolan., et al. “Avian egg antibodies: basic and potential applications”. Avian and Poultry Biology Reviews 15.1 (2004): 25-46.
  7. Mishra Neeraj., et al. “Edible vaccines: A new approach to oral immunization”. (2008).
  8. Sack David A., et al. “Antimicrobial resistance in shigellosis, cholera and campylobacteriosis”. No. WHO/CDS/CSR/DRS/2001.8. Geneva: World Health Organization (2001).
  9. Newell Diane G., et al. “Food-borne diseases—the challenges of 20 years ago still persist while new ones continue to emerge”. International Journal of Food Microbiology 139 (2010): S3-S15.
  10. Alam Nure H and Hasan Ashraf. "Treatment of infectious diarrhea in children”. Pediatric Drugs 5.3 (2003): 151-165.
  11. Mani Sachin., et al. “Status of vaccine research and development for Shigella”. Vaccine 34.26 (2016): 2887-2894.
  12. Kaminski RW., et al. “Development and preclinical evaluation of a trivalent, formalin-inactivated Shigella whole-cell vaccine”. Clinical and Vaccine Immunology 21.3 (2014): 366-382.
  13. Schade R., et al. “Polyclonal IgY antibodies from chicken egg yolk--an alternative to the production of mammalian IgG type antibodies in rabbits”. Alternatives to laboratory animals: ATLA (1991).
  14. Kurien Biji T and R Hal Scofield. “Protein electrophoresis: methods and protocols”. Totowa, NJ: Humana Press (2012).
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Citation

Citation: Prakash Narayana Reddy., et al. “IgY: A Promising Alternative to IgG in Immunodiagnostics".Acta Scientific Biotechnology 1.1 (2020): 18-21.



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