Abstract

  The immune system is influenced by various internal (like hormones) and external (environmental: seasons) factors in which melatonin plays a vital role. Melatonin modulates seasonal immunity by influencing the cell-mediated immune function like cytokine secretion and cellular proliferation in rodents, however, there is a lack of information that how melatonin modulates the secretion of different cytokines in different seasons and directs the cellular proliferation in presence of specific cytokine. Therefore, in the present study, we elucidated the effect of seasonal melatonin variation on peripheral cytokines (IL-2, IL-6, and TNF-α) level and proliferative response (%SR) of peripheral blood mononuclear cells (PBMCs). The effect of recombinant cytokines (rIL-2, rIL-6, and rTNF-α) on the proliferative response of PBMCs was also noted in presence of melatonin under in vitro condition. As a result, we observed that high endogenous melatonin causes an increased level of IL-2, and IL-6 and down-regulates TNF-α during the winter season. Melatonin significantly modulates the proliferative response of PBMCs in presence of rIL-2, rIL-6 and rTNF-α by enhancing the immunomodulatory effect of rIL-2 and rIL-6 and by diminishing immunosuppressive effect of rTNF-α in goat. In conclusion, we may propose that melatonin maintains immune-homeostasis by modulating circulatory cytokines levels during different seasons. Melatonin might influence the secretion of immunomodulatory cytokine (i.e. IL-2 and IL-6) and also regulate the immuno-inhibitory action of TNF-α to modulate cell-mediated immune function.

Keywords: Season; Melatonin; Cytokines; Recombinant Cytokines; Ruminant; Immune Function

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Citation

Citation: Amaresh Kumar Singh and Chandana Haldar. “Seasonal Cytokine Production and Combinatorial Effect of Recombinant Cytokines and Melatonin on Peripheral Blood Mononuclear Cells Proliferation”Acta Scientific Medical Sciences 2.9 (2020): 06-11.

Copyright

Copyright: © 2020 Amaresh Kumar Singh and Chandana Haldar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.