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Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 4 Issue 10

Phytochemical Analysis, Antioxidant and Antiarthritic Activity of Aqueous Extracts of Gmelina arborea Flowers

Ayushi Rajput, Sourabh Jain* and Karunakar Shukla

College of Pharmacy, Dr. A. P. J. Abdul Kalam University, Indore, MP, India

*Corresponding Author: College of Pharmacy, Dr. A. P. J. Abdul Kalam University, Indore, MP, India

Received: September 02, 2020; Published: September 28, 2020

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Abstract

  The plant Gmelina arborea (G. arborea) has been traditionally used in India for several medicinal purposes like anthelmintic, diuretic, antibacterial, antioxidant and antidiabetic. The aim of the present study was to evaluate qualitative phytochemical analysis, antioxidant and antiarthritic activities of aqueous extracts of G. arborea flowers. Qualitative analysis of various phytochemical constituents was determined by the well-known test protocol available in the literature. The in vitro antioxidant activity was assessed against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide scavenging assay using standard protocols. The in vitro antiarthritic potential of extracts was evaluated in terms of its inhibition of protein denaturation and membrane stabilization method. Freund’s adjuvant arthritis model in Wistar rats was used to study the in vivo antiarthritic activity. Phytochemical analysis revealed the presence of alkaloids, glycosides, phenols, flavonoids, tannins. The inhibitory concentration 50% values are 85.43 and 107.59 µg/ml for DPPH radical scavenging assay and superoxide scavenging assay, respectively. The plant extracts exhibited dose dependent inhibition of protein denaturation and also protected the RBC cells by stabilizing the membranes. G. arborea flowers extracts (100 and 200 mg/kg) was given orally to arthritic rats induced with complete freund’s adjuvant and changes in paw diameter, joint diameter, arthritic score and body weight were determined. Indomethacin was taken as standard. Rats treated with extracts displayed marked reduction in paw diameter, joint diameter and arthritic score along with substantial enhancement in body weight. The antiarthritic effects of these plant extracts may attribute either due to its inhibition of protein denaturation or stabilizing the membranes from the free radical attack which are generated due to the immunological and inflammatory reactions observed in most of the arthritic conditions.

Keywords: Gmelina arborea; Phytochemical Analysis; Antioxidant; Antiarthritic Activity

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References

  1. Zawadzka-Bartczak E., et al. “Antioxidative enzyme profiles in fighter pilots”. Aerospace Medicine and Human Performance 74 (2003): 654-658.
  2. Deshpade V and Jadhav M. “Invitro anti-arthritic activity of Abution indicum”. Journal of Pharmaceutical Research 4 (2009): 644-645.
  3. Feldmann M., et al. “Rheumatoid arthritis”. Cell3 (1996): 307-310.
  4. Nadkarni KM. “Indian Materia Medica”. Mumbai: Popular Prakashan (2000): 584-585.
  5. Kirtikar KR and Basu BD. “Indian Medicinal Plants”. Dehradun: International Book Distributors (1999): 1932-1933.
  6. Khan IA and Khanum A. “Herbal Therapy for Diabetes”. Hyderabad: Ukaaz Publications (2005): 34-35.
  7. Rao DV., et al. “Occurrence of luteolin in the leaves of Gmelina arborea Linn”. Current Science 3 (1967): 71-74.
  8. Bhattacharjee AK and Das AK. “Phytochemical survey of few mysore Plants”. Economic Botany 23 (1969): 274-276.
  9. Hosny M., et al. “Twelve acylated iridoid glycosides from Gmelina arborea”. Journal of Natural Products 61 (1998): 734-742.
  10. Joshi KC., et al. “Extractives from heartwood”. Journal of the Indian Chemical Society 48 (1971): 1175-1176.
  11. Govindachari TR., et al. “Arboreol, A new lignan from Gmelina arborea”. Indian Journal of Chemistry 10 (1972): 1120-1122.
  12. Anjaneyulu AS., et al. “The structures of lignans from Gmelina arborea”. Tetrahedron 31 (1975): 1277-85.
  13. Satyanarayana P., et al. “Arborone and 7-Oxo-dihydrogmelinol: Two New Keto-Lignans from Gmelina arborea”. Journal of Natural Products 49 (1986): 1061-1064.
  14. Shirwaikar A., et al. “Effects of Gmelina arborea Roxb leaves on wound healing in rats”. Journal of Natural Remedies 3 (2003): 45-48.
  15. Agunua A., et al. “Evaluation of five medicinal plants used in diarrhoea treatment in Nigeria”. Journal of Ethnopharmacology 101 (2005): 27-30.
  16. Sinha S., et al. “Bark and fruit extracts of Gmelina arborea protect liver cells from oxidative stress”. Pharmaceutical Biology 44 (2006): 237-243.
  17. Deepak Kumar Jain., et al. “Appraisal of in vitro Antioxidant and in vivo Anti-Inflammatory Activities of Various Extracts from the Fruits of Vitis vinifera L”. Scholars Academic Journal of Pharmacy 3 (2019): 86-93.
  18. Deepak Kumar Jain., et al. “Anti-arthritic activity of tridax procumbens ethanolic extract of leaves”. RGUHS Journal of Pharmaceutical Sciences 4 (2012): 80-86.
  19. Ramya P and Lakshmidevi N. “Studies on anti-oxidant activity of Tinospora cardifolia (Miers)”. Journal of American Science 10 (2010): 736-743.
  20. Neha P and Dushyant B. “Antioxidant activity of ethanolic extract of Annona squamosa Linn bark”. International Journal of Research in Pharmaceutical and Biomedical Sciences 2 (2011): 1692-1697.
  21. Veerapur VP., et al. “Ficus racemosa stem bark extract: A potent antioxidant and a probable natural radioprotector”. Evidence-Based Complementary and Alternative Medicine 3 (2009): 317-324.
  22. Badii F and Howell NK. "Effect of antioxidants, citrate, and cryoprotectants on protein denaturation and texture of frozen cod (Gadus morhua)”. Journal of Agricultural and Food Chemistry 50 (2002): 2053-2061.
  23. Manukumar HM and Umesha S. "Assessment of membrane stabilizing activity from honey. An in-vitro approach". Acta Scientiarum Polonorum, Technologia Alimentaria 14 (2015): 85-90.
  24. Chung JI., et al. “Anti-inflammatory effect of low intensity ultrasound (LIUS) on complete Freund's adjuvant-induced arthritis synovium". Osteoarthritis Cartilage 20 (2012): 314-322.
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Citation

Citation: Sourabh Jain., et al. “Phytochemical Analysis, Antioxidant and Antiarthritic Activity of Aqueous Extracts of Gmelina arborea Flowers". Acta Scientific Pharmaceutical Sciences 4.10 (2020): 165-171.



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