Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 4 Issue 10

Simultaneous Estimation of Acebrophylline, Montelukast Sodium and Levocetirizine by RP-UPLC Method in Combined Dosage Forms

S Sangeetha1*, S Alexandar2 and B Jaykar2

1Department of Pharmaceutical Chemistry, Vinayaka Missions College of Pharmacy, Salem, India
2Vinayaka Mission’s Research Foundation (Deemed to be University), Tamil Nadu, India

*Corresponding Author: S Sangeetha, Lecturer, Department of Pharmaceutical Chemistry, Vinayaka Missions College of Pharmacy, Salem and Vinayaka Mission’s Research Foundation (Deemed to be University), Tamil Nadu, India.

Received: April 04, 2020; Published: September 18, 2020

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Abstract

A new simple, precise method was developed for Acebrophylline, Montelukast sodium and Levocetirizine by using UPLC for Simultaneous Estimation. Acebrophylline is combination of Theophylline and Ambroxol. The compounds were separated by using column C- 18 BEH 2.1 x 50 mm (1.7 µm) at ambient temperature. Flow rate was 0.6 ml/min; wavelength of 230 nm, Mobile phase was methanol:acetonitrile: Buffer (30:40:30) potassium dihydrogen phosphate buffer was used with pH 6.0. The injection volume was 2 µl and Run time 4.5 min. The retention time of Acebrophylline (Theophylline and Ambroxol), Montelukast sodium and Levocetirizine 0.87,1.34, 2.01, 3.20 mins respectively. The percentage purity was found to be 99.72, 99.96 and 99.81 respectively. The validation parameters Specificity, Accuracy, linearity, LOD, LOQ and Robustness were studied. The linearity of was Acebrophylline 40 - 320 µg/ml, for Montelukast sodium 2 - 16 µg/ml and for Levocetirizine 1-8 µg/ml They have good linearity with Correlation coefficient Value of 0.996, 0.994, 0.994 respectively.

Keywords: UPLC; Acebrophylline; Montelukast Sodium and Levocetirizine; Simultaneous Estimation

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References

  1. Sharma Bhavik and Agarwal Sushil Kumar. “RP-HPLC Method Development and Validation for Estimation of Acebrophylline”. Asian Journal of Pharmaceutical Research and Development 6.6 (2018): 56-59.
  2. Mittal M., et al. “Simultaneous estimation of acebrophylline, montelukast, and levocetirizine dihydrochloride in marketed formulation by high-performance liquid chromatography method”. Pharma Aspire 10.1 (2018): 23-28. 
  3. Patil SS., et al. “Development and statistical validation of spectrophatometry method for estimation of Montelukast in bulk and tablet dosage form”. Journal of Pharmacy Research 2.4 (2009): 714-716. 
  4. Singh RM., et al. “Development and validation of a RP-HPLC method for estimation of montelukast sodium in bulk and in tablet dosage form”. Indian Journal of Pharmaceutical Sciences 72.2 (2010): 235-237.
  5. Choudhari V., et al. “Simultaneous determination of montelukast sodium and levocetirizine dihydrochloride in pharmaceutical preparations by ratio derivative spectroscopy”. International Journal of PharmTech Research (2010).
  6. Ali OI., et al. “Validated derivative and ratio derivative spectrophotometric methods for the simultaneous determination of levocetirizine dihydrochloride and ambroxol hydrochloride in pharmaceutical dosage form”. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 153 (2016): 605-611.
  7. RM Singh., et al. “Development and Validation of a RP-HPLC Method for Estimation of Montelukast Sodium in Bulk and in Tablet Dosage Form”. Indian Journal of Pharmaceutical Sciences 72 (2010).
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Citation

Citation: S Sangeetha., et al. “Simultaneous Estimation of Acebrophylline, Montelukast Sodium and Levocetirizine by RP-UPLC Method in Combined Dosage Forms". Acta Scientific Pharmaceutical Sciences 4.10 (2020): 52-55.




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Impact Factor0.759

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