Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 4 Issue 2

The Potential of Emamectin Benzoate to Induce Kidney DNA Oxidation, Heat Shock Protein Levels and Apoptosis in Male Mice

Ozge Temiz*

Department of Biology, Faculty of Science and Letters, Cukurova University, Turkey

*Corresponding Author: Ozge Temiz, Department of Biology, Faculty of Science and Letters, Cukurova University, Turkey.

Received: January 18, 2020; Published: January 23, 2020



  Biopesticide Emamectin benzoate (EMB), which is used in a wide range of areas such as food production and household pest removal, causes toxic effects caused by different uses and can cause health problems in human life. As a result of pesticide exposure, it causes biological damage such as oxidative toxicity and initiation of apoptosis signal and genotoxic effects. In sublethal EMB doses; E1 (25 mg/kg/day), E2 (50 mg/kg/day), E3 (100 mg/kg/day) were administered to Swiss albino male mice for 14 days by gavage. Male mice in kidney tissues; changes in thiobarbituric acid reagent (TBARS) levels, HSP70 (heat shock proteins 70) levels known as stress protein, apoptosis marker caspase 3 enzyme activity and DNA oxidation marker 8-hydroxy-2-deoxyguanosine (8-OHdG) levels were investigated. The results of this study showed that the increase in TBARS level in kidney tissue after exposure to EMB caused lipid peroxidation. An increase in HSP70 levels were determined due to the stress response resulting from the toxicity of the tissues of the EMB groups. In addition, increases in caspase 3 enzyme activation and 8-OHdG levels were determined to cause both apoptosis and DNA oxidation. These changes in different molecular toxicology parameters indicate that the toxicity of EMB is effective in the kidneys of male mice at 14 days of exposure.

Keywords: Emamectin Benzoate; Caspase 3; HSP70; 8-OhdG; Mice; Kidney



  1. Furlan Lorenzo and David Kreutzweiser. "Alternatives to neonicotinoid insecticides for pest control: case studies in agriculture and forestry”. Environmental Science and Pollution Research 22.1 (2015): 135-147.
  2. Souza Andressa de., et al. "Evaluation of the impact of exposure to pesticides on the health of the rural population: Vale do Taquari, State of Rio Grande do Sul (Brazil)”. Ciencia and Saude Coletiva 16.8 (2011): 3519-3528.
  3. Mostafalou Sara., et al. "Protective effect of NAC against malathion-induced oxidative stress in freshly isolated rat hepatocytes”. Advanced pharmaceutical bulletin 2.1 (2012): 79.
  4. Özge Temiz“Emamectin Benzoate - Induced Oxidative Stress, Heat Shock Protein Levels and Apoptotic Effects in the Brain of Male Mice”. Acta Scientific Veterinary Sciences 2.2 (2020): 01-04.
  5. Geary Timothy G. "Ivermectin 20 years on: maturation of a wonder drug”. Trends in parasitology 21.11 (2005): 530-532.
  6. Üner Nevin., et al. “Piperonyl butoxide increases oxidative toxicity of fenthion in the brain of Oreochromis niloticus”. Journal of Biochemical and Molecular Toxicology 28.2 (2014): 84-90.
  7. Van der Oost., et al. "Fish bioaccumulation and biomarkers in environmental risk assessment: a review”. Environmental toxicology and pharmacology 13.2 (2003): 57-149.
  8. Zhao Rongmin and Walid A Houry. "Molecular interaction network of the Hsp90 chaperone system”. Molecular Aspects of the Stress Response: Chaperones, Membranes and Networks. Springer, New York, NY (2007): 27-36.
  9. Porter Alan G and Reiner U Jänicke. "Emerging roles of caspase-3 in apoptosis”. Cell death and differentiation 6.2 (1999): 99.
  10. Ghavami Saeid., et al. "Apoptosis and cancer: mutations within caspase genes”. Journal Of Medical Genetics 46.8 (2009): 497-510.
  11. Nakae Dai., et al. "Age and organ dependent spontaneous generation of nuclear 8-hydroxydeoxyguanosine in male Fischer 344 rats”. Laboratory Investigation 80.2 (2000): 249.
  12. Collins, Andrew R. "Investigating oxidative DNA damage and its repair using the comet assay”. Mutation Research/Reviews in Mutation Research 681.1 (2009): 24-32.
  13. Wolterink G., et al. "Emamectin benzoate”. Pesticide Residues in Food—2011 (2012): 211.
  14. Bradford Marion M. "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding”. Analytical biochemistry 72.1-2 (1976): 248-254.
  15. De Boeck Gudrun., et al. "Effect of cortisol treatment and/or sublethal copper exposure on copper uptake and heat shock protein levels in common carp, Cyprinus carpio”. Environmental Toxicology and Chemistry: An International Journal 22.5 (2003): 1122-1126.
  16. Ohkawa Hiroshi., et al. "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction”. Analytical biochemistry 95.2 (1979): 351-358.
  17. Yin Baoyun., et al. "Determination of 8-hydroxydeoxyguanosine by an immunoaffinity chromatography-monoclonal antibody-based ELISA”. Free Radical Biology and Medicine 18.6 (1995): 1023-1032.
  18. Turman Martin A and Scott L Rosenfeld. "Heat shock protein 70 overexpression protects LLC-PK1 tubular cells from heat shock but not hypoxia”. Kidney international 55.1 (1999): 189-197.
  19. Franco Jeferson L., et al. "Zinc reverses malathion-induced impairment in antioxidant defenses”. Toxicology Letters 187.3 (2009): 137-143.
  20. Makeen Hafiz Antar., et al. "Cytoprotective effect of Cactus cladode (Opuntia ficus-indica) against chlorpyrifos induced reactive oxygen species in rat hepatocytes: Involvement of heat shock protein 70 and CYP1A1/2 proteins”. Pharmacognosy Magazine 15.62 (2019): 47.
  21. Lonare Milindmitra., et al. "Evaluation of ameliorative effect of curcumin on imidacloprid‐induced male reproductive toxicity in wistar rats”. Environmental toxicology 31.10 (2016): 1250-1263.
  22. Badgujar Prarabdh C., et al. "Fipronil induced oxidative stress involves alterations in SOD 1 and catalase gene expression in male mice liver: Protection by vitamins E and C”. Environmental toxicology 31.9 (2016): 1147-1158.
  23. Badgujar Prarabdh C., et al. "Fipronil induced oxidative stress in kidney and brain of mice: protective effect of vitamin E and vitamin C”. Pesticide biochemistry and physiology 118 (2015): 10-18.
  24. Schneider Claus., et al. "Routes to 4-hydroxynonenal: fundamental issues in the mechanisms of lipid peroxidation”. Journal of Biological Chemistry 283.23 (2008): 15539-15543.
  25. Sayed Amira A., et al. "Garlic and allopurinol attenuate hepatic apoptosis induced by fipronil in male albino rats”. Regulatory Toxicology and Pharmacology (2019).
  26. Jestadi Dinesh Babu., et al. "Effects of short term exposure of atrazine on the liver and kidney of normal and diabetic rats”. Journal of toxicology (2014).
  27. Saquib Quaiser., et al. "Phorate-induced oxidative stress, DNA damage and transcriptional activation of p53 and caspase genes in male Wistar rats”. Toxicology and applied pharmacology 259.1 (2012): 54-65.
  28. Kijima Kazuyasu., et al. "Gene expression analysis of the rat testis after treatment with di (2-ethylhexyl) phthalate using cDNA microarray and real-time RT-PCR”. Toxicology and applied pharmacology 200.2 (2004): 103-110.
  29. You Li and Erika J Bartolucci. "Gene expression profiles in mammary gland of male rats treated with genistein and methoxychlor”. Environmental toxicology and pharmacology 18.2 (2004): 161-172.
  30. Green Douglas R and Gustavo P Amarante-Mendes. "The point of no return: mitochondria, caspases, and the commitment to cell death”. Apoptosis: Mechanisms and Role in Disease. Springer, Berlin, Heidelberg (1998): 45-61.
  31. Mohasseb Magda., et al. "Testicular oxidative damage and role of combined antioxidant supplementation in experimental diabetic rats”. Journal of physiology and biochemistry 67.2 (2011): 185-194.
  32. Shi Yigong. "Mechanisms of caspase activation and inhibition during apoptosis”. Molecular cell 9.3 (2002): 459-470.
  33. Boiteux Serge and J Pablo Radicella. "The human OGG1 gene: structure, functions, and its implication in the process of carcinogenesis”. Archives of biochemistry and biophysics 377.1 (2000): 1-8.
  34. Clayson David B., et al. "Oxidative DNA damage—the effects of certain genotoxic and operationally non-genotoxic carcinogens”. Mutation Research/Reviews in Genetic Toxicology 317.1 (1994): 25-42.
  35. Bolognesi Claudia., et al. "Genotoxic activity of glyphosate and its technical formulation Roundup”. Journal of Agricultural and food chemistry 45.5 (1997): 1957-1962.


Citation: Ozge Temiz. “The Potential of Emamectin Benzoate to Induce Kidney DNA Oxidation, Heat Shock Protein Levels and Apoptosis in Male Mice". Acta Scientific Pharmaceutical Sciences 4.2 (2020): 55-59.

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