Rafia Sameen1 and Shakira Ghazanfar2*
1Department of Animal Genomics and Biotechnology, PIASA National Agriculture
Research Centre, Islamabad, Pakistan
2National Institute of Genomics and Agriculture Biotechnology (NIGAB), National Agriculture Research Centre, Islamabad, Pakistan
*Corresponding Author: Shakira Ghazanfar, National Institute of Genomics and Agriculture Biotechnology (NIGAB), National Agriculture Research Centre, Islamabad, Pakistan.
Received: May 22, 2020; Published: June 19, 2020
Aviculture is the efficient animal production system and good source of animal protein worldwide. Poultry gastrointestinal tract houses certain microbial communities with bacteria being dominant above all. These bacteria produce beneficial products and result in non-pathogenic immune response providing nutrition and protection for animals. Antibiotic treatment causes reduction of beneficial bacterial population in intestine which can be controlled by probiotic supplements. Probiotics play their role to control intestinal pathogens by competing for adhesion sites and nutrients, producing anti-bacterial substances. Lactic acid bacteria could be a good probiotic for animal use among which Lactobacillus fermentum is major heterofermentative specie found to have probiotic potential and can be used in supplements for animal feed. Its probiotic potential was well studied by its tolerance to inhibitory substances like bile and salt, its antimicrobial activity and evaluation by supplementing it in poultry feed. To identify probiotic properties of Lactobacillus fermentum, its whole-genome was sequenced and analysed. Whole genome sequencing is a DNA sequencing technology that has revolutionized genomic research. Whole genome is sequenced, assembled and annotated using bioinformatics tool and then analyzed. Galaxy is one of most commonly used genome analysis tool that provides data analysis support through framework, to give simple interfaces to certain powerful tools and automatically manage computational details. Following to evaluation of probiotic potential through certain parameters and whole genome sequencing, antimicrobial resistance can also be evaluated to make a decision about safety of L. fermentum use as probiotic in poultry feed. Administration of L. fermentum to poultry showed beneficial effects on their growth. Introduction
Keywords: Probiotics; Lactobacillus fermentum; Whole Genome Sequencing (WGS); Galaxy
Citation: Rafia Sameen and Shakira Ghazanfar. “Whole-Genome Sequencing of Lactobacillus fermentum and its Application as Probiotic in Poultry Feed". Acta Scientific Nutritional Health 4.7 (2020): 49-54.
Copyright: © 2020 Rafia Sameen and Shakira Ghazanfar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.