Abstract

  The biodegradation of crude oil through biosurfactant produced by bacteria isolated from oily sludge collected from contaminated sites of three different refineries were studied. Eight bacterial isolates were screened for biosurfactant production by serial dilution technique and spread plate method. Two bacterial isolates were found capable of producing significant quantity of biosurfactant exhibited by surface tension activity (26.66 dy/cm), emulsification activity (up to 79%) and total petroleum hydrocarbon reduction (up to 92%). Gas chromatography analysis of 5 days treated MSM containing 1% crude oil as sole source of carbon and energy showed decrease in the concentration of aliphatic and aromatic hydrocarbon present in crude oil. The degradation of aliphatic and aromatic compounds of crude oil clearly indicates the production of biosurfactant by both bacterial isolates (TER-4 and TER-7).

Keywords: Biodegradation; Crude Oil; Biosurfactant; Isolate; Oily Sludge

References

1. Bhattacharya D., et al. “Evaluation of genetic diversity among Pseudomonas citronellolis strains isolated from oily sludge contaminated sites”. Applied and Environmental Microbiology 69 (2003): 1431-1441.
2. Mandal AK., et al. “Bioremediation: an environment friendly sustainable biotechnological solution for remediation of petroleum hydrocarbon contaminated waste”. International Journal of Research in Engineering, IT and Social Sciences 2.8 (2012): 1-18.
3. Xia W., et al. “Biosurfactant produced by novel Pseudomonas sp. WJ6 with biodegradation of n-alkanes and polycyclic aromatic hydrocarbons”. Journal of Hazardous Materials 276 (2014): 489-498.
4. Mohan PK., et al. “Biokinetics of biodegradability of surfactants under aerobic, anoxic and anaerobic conditions”. Water Research 40 (2006): 533-540.
5. Mulligan CN. "Environmental applications for biosurfactants”. Environmental Pollution 133.2 (2005): 183-198.
6. Lal B and Khanna S. "Degradation of crude oil by Acinetobacter calcoaceticus and Alcaligenes odorans". Journal of Applied Bacteriology 81 (1996): 355-362.
7. Bodour AA and Miller-Maier R. "Application of a modified drops collapse technique for surfactant quntitation and screening of biosurfactant producing microorganisms”. Journal of Microbiological Methods 32 (1998): 273-280.
8. Cooper DG and Goldenberg BG. "Surface-Active agents from two Bacillus species”. Applied and Environmental Microbiology 53 (1987): 224-229.
9. Mishra Sanjeet., et al. “Evaluation of Inoculum Addition To Stimulate In Situ Bioremediation of Oily-Sludge-Contaminated Soil”. Applied and Environmental Microbiology 67.4 (2011): 1675-1681.
10. Luque de Castro MD and Priego-Capote F. "Soxhlet extraction: past and present panacea”. Journal of Chromatography A 1217 (2010): 2383-2389.
11. Saisa-Ard K., et al. “Isolation and characterization of biosurfactants producing bacteria isolated from palm oil industry and evaluation for biosurfactants production using low-cost substrates". Journal of Chemical Technology and Biotechnology 94 (2013): 275-284.
12. Mnif S., et al. “Simultaneous hydrocarbon degradation and biosurfactant production by oilfield selected bacteria”. Journal of Applied Microbiology 111(2011): 525-536.
13. Kumari S., et al. “Improved polycyclic aromatic hydrocarbon degradation in a crude oil by individual and a consortium of bacteria”. Bioresource Technology 254 (2018): 174-179.
14. Marchant R and Banat IM. "Microbial biosurfactants: challenges and opportunities for future exploitation”. Trends in Biotechnology 30 (2012): 558-565.
15. Kavitha V., et al. “Microbial biosurfactant mediated removal and/or solubilization of crude oil contamination from soil and aqueous phase: an approach with Bacillus licheniformis MTCC 5514”. International Biodeterioration and Biodegradation 94 (2014): 24-30.
16. Tahseen R., et al. “Rhamnolipids and nutrients boost remediation of crude oil-contaminated soil by enhancing bacterial colonization and metabolic activities”. International Biodeterioration and Biodegradation 115 (2016): 192-198.
17. Martins PC and Martins VG. "Biosurfactant production from industrial wastes with potential remove of insoluble paint”. International Biodeterioration and Biodegradation 127 (2018): 10-16.
18. Li J., et al. “Production and characteristics of biosurfactant produced by Bacillus pseudomycoides BS6 utilizing soybean oil waste”. International Biodeterioration and Biodegradation 112 (2016): 72-79.
19. Shekhar S., et al. “Biosurfactant producing microbes and their potential applications: a review”. Critical Reviews in Environmental Science and Technology 45 (2015): 1522-1554.
20. Jimoh AA and Lin J. "Production and characterization of lipopeptide biosurfactant producing Paenibacillus sp. D9 and its biodegradation of diesel fuel”. International Journal of Environmental Science and Technology 16 (2019): 4143-4158.
21. De Franca IWL., et al. “Production of a biosurfactant by Bacillus subtilis ICA56 aiming bioremediation of impacted soils”. Catalysis Today 255 (2015): 10-15.
22. Anyanwu C., et al. “Lipopeptide biosurfactant production by Serratia marcescens NSK-1 strain isolated from petroleum-contaminated soil”. Journal of Applied Sciences Research 7 (2011): 79-87.
23. Darvishi P., et al. “Biosurfactant production under xtreme environmental conditions by an efficient microbial consortium, ERCPPI-2”. Colloids Surfaces B Biointerfaces 84 (2011): 292-300.
24. Janek T., et al. “Isolation and characterization of two new lipopeptide biosurfactants produced by Pseudomonas fluorescens BD5 isolated from water from the Arctic Archipelago of Svalbard”. Bioresource Technology 101 (2011): 6118-6123.
25. Lima TM., et al. “Evaluation of bacterial surfactant toxicity towards petroleum degrading microorganisms”. Bioresource Technology 102 (2011): 2957-2964.

Citation

Citation: Shaili Srivastava.,et al.“Biodegradation of Crude Oil Through Biosurfactant Producing Bacterial Strains Isolated from Oily Sludge". Acta Scientific Microbiology 3.3 (2020): 01-06.

Copyright

Copyright: © 2020 Shaili Srivastava.,et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.