Abstract

  Rotavirus is the most common cause of diarrhoeal disease among infants and young children. It is a genus of double-stranded RNA viruses in the family Reoviridae. According to literatures, nearly every child in the world is infected with rotavirus at least once by the age of five. The study was carried out to determine the incidence of Rotavirus among children (0 - 5years) suffering from diarrhoea attending Hasiya Bayero Pediatric Hospital, Kano state. Two hundred and ninety-nine children within ages 0 - 5 years who were at Hasiya Bayero Pediatric Hospital, Kano, at the time of the study were enrolled in the study. Diarrhoea samples were obtained following parental consent and ethical approval from the medical research ethics committee of the hospital. The stool samples were collected aseptically in sterile commercial stool containers adequately labeled (patient ID and date of collection) and were transported in ice to the Center for Biotechnology Research, Bayero University Kano where they we restored at −200C until tested. The presence of Rotavirus was detected using RDT and ELISA (IgM) in the stool samples. Rotavirus (IgM) was detected in 22 of the 299 subjects giving an incidence of 7.4% using ELISA. The RDT kit detected Rotavirus in 45 of the 299 subjects giving an incidence of 15.1%. Female subjects had higher incidence (51.1%) compared to male subjects (48.9%). Subjects in age group 13-24 months old had the highest incidence of Rotavirus (5.0% and 3.7%) while subjects in age group 25 - 36 months old had the lowest percent positive (0.7% and 0.3%) for RDT and ELISA respectively. The socio-demographic variables associated with the incidence of Rotavirus in this study were mother’s level of education, breastfeeding practices and method of water storage. ELISA test kit was found to be more specific and sensitive when compared with Rapid test kit used in this study in detecting Rotavirus. It is recommended that mothers should be educated through their anti-natal and post-natal clinics on the process of avoiding the infection through effective hygiene especially when babies are between 0-5years old especially 13 - 24 months and the detection of Rotavirus among diarrhoea patients should focus more on the use of ELISA instead of RDT.

Keywords: Rotavirus; Diarrhoea; Children

References

1. Dennehy PH. "Rotavirus Infection: A Disease of the Past?”. Infectious Disease Clinics of North America 29.4 (2015): 617-635. 
2. Bernstein DI. "Rotavirus overview”. The Pediatric Infectious Disease Journal 28.3 (2009): S50-S53.
3. Grimwood K and Lambert SB. "Rotavirus vaccines: opportunities and challenges”. Human Vaccines 5.2 (2009): 57-69. 
4. Bishop R. "Discovery of Rotavirus: Implications for child health”. Journal of Gastroenterology and Hepatology 24.3 (2009): S81-S85. 
5. World Health Organization. "Global Rotavirus Sentinel Hospital Surveillance Network" (PDF) (2015).
6. Dubovi EJ and Mac Lachlan NJ. “Fenner's Veterinary Virology (4th ed.)”. Boston: Academic Press (2010): 288. 
7. Fischer TK., et al. “Hospitalizations and deaths from diarrhea and Rotavirus among children <5 years of age in the United States, 1993–2003”. Journal of Infectious Diseases195.8 (2007): 1117-1125. 
8. Leshem E., et al. “Rotavirus Vaccines and Health Care Utilization for Diarrhea in the United States”. Pediatrics 134.1 (2014): 15-23.
9. Tate JE., et al. “Uptake, impact, and effectiveness of Rotavirus vaccination in the United States: review of the first 3 years of postlicensure data”. The Pediatric Infectious Disease Journal 30 (2011): S56-S60. 
10. Diggle L. "Rotavirus diarrhea and future prospects for prevention”. British Journal of Nursing 16.16 (2007): 970-974. 
11. Giaquinto C., et al. “Summary of effectiveness and impact of Rotavirus vaccination with the oral pentavalent rotavirus vaccine: a systematic review of the experience in industrialized countries”. Human Vaccines 7.7 (2011): 734-748. 
12. Jiang V., et al. “Performance of Rotavirus vaccines in developed and developing countries”. Human Vaccines 6.7 (2010): 532-542. 
13. Parashar UD and Tate JE. "Health Benefits of Rotavirus Vaccination in Developing Countries”. Clinical Infectious Diseases 62.2 (2016): S91-228. 
14. Kapikian AZ., et al. “Rotaviruses”. In Fields virology. 4th ed. Philadelphia (PA): Lippincott Williams and Wilkins (2001): 1787-1833.
15. Tate JE., et al. “Global, Regional, and National Estimates of Rotavirus Mortality in Children <5 Years of Age, 2000-2013”. Clinical Infectious Diseases 62.2 (2016): S96-105.
16. American Academy of Pediatrics. “Rotavirus infections”. In: Pickering L. K, editor. Red Book: 2003 report of the Committee on Infectious Diseases. 26th ed. Elk Grove Village, IL: American Academy of Pediatrics (2003): 534-536.
17. Kotloff KL., et al. “Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study”. Lancet 382.9888 (2013): 209-222.
18. Moutelikova., et al. “Human rotavirus A detection: Comparison of enzymatic immunoassay and rapid chromatographic test with two quantitative RT-PCR assays”. Epidemiologie, mikrobiologie, imunologie, Praha: Česká lékařská společnost Jana Evangelisty Purkyně, 67.3 (2018):110-113. 
19. Monica and David., et al. Vaccines for Biodefense and Emerging and Neglected Diseases (2009).

Citation

Citation: Suleiman Kafilat Olayemi and Usman Aliyu Dutsinma. “Incidence of Rotavirus Among Children with Diarrhoea Attending Hasiya Bayero Pediatric Hospital, Kano State, Nigeria". Acta Scientific Microbiology 3.3 (2020): 01-08.

Copyright

Copyright: © 2020 Suleiman Kafilat Olayemi and Usman Aliyu Dutsinma. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.