Anargyros Skoulakis¹, Vasileios Lachanas², Zoe Florou¹, Maria Samara³, Myrto Mantzana-Petinelli ¹, Efthymia Petinaki¹ and Charalampos Skoulakis²*

¹Department of Microbiology, School of Medicine, University of Thessaly, Larissa, Greece
²Department of Otorhinolaryngology, University Hospital of Larissa, Greece
³Department of Pathology, School of Medicine, University of Thessaly, Greece

*Corresponding Author: Charalampos Skoulakis, Associate Professor, Department of Otorhinolaryngology, University Hospital of Larissa, Greece.

Received: February 09, 2018; Published: March 03, 2018

DOI: 10.31080/ASMI.2018.01.0031

Citation: Charalampos Skoulakis., et al. “High-Prevalence of Various High-Risk Subtypes of Human Papilloma Virus in Patients with Acquired Cholesteatoma in Greece”. Acta Scientific Microbiology 1.4 (2018).

Abstract

  Although there are a lot of theories, the pathogenesis of cholesteatoma remains unclear. Few studies exist worldwide regarding the presence of Human Papilloma Virus (HPV) in cholesteatoma with controversial results. The presence of DNA of various high and low risk HPV sub-types was detected by real-time PCR to lesions obtained from sixty-two patients with acquired cholesteatoma. In addition, the presence of bacterial DNA was also detected using 16S rRNA PCR. Control group included tympanic membrane skin samples from patients operated by tympanoplasty. Cholesteatoma samples of thirty patients (48.3%) were found to be positive for one at least HPV sub-type. Twenty-five samples were positive for HPV16 (40.3%), nine were positive for HPV6 (14.5%), one for HPV18 (1.6%), one for HPV31 (1.6%), one for HPV51 (1.6%) and one for HPV59 (1.6%). None of the patients of control group was found to be positive for any sub-type HPV (0%). On the other hand, all specimens (cholesteatoma and tympanic membrane skin mu- cosa) were negative for the presence of bacterial DNA. Our results demonstrate a high prevalence of various high and low-risk HPV sub-types in cholesteatoma tissue samples in Central Greece.

Keywords: HPV; sub-types acquired holesteatoma; Greece

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