Dalia S Ashour*, Ibrahim A Aboul Assad, Samy I El-Kowrany and Amira E Abdel Ghaffar

Department of Medical Parasitology, Faculty of Medicine, Tanta University, Tanta, Egypt

*Corresponding Author: Dalia S Ashour, Associate Professor, Department of Medical Parasitology, Faculty of Medicine, Tanta University, Tanta, Egypt.

Received: December 11, 2017; Published: January 30, 2018

DOI: 10.31080/ASMI.2018.01.0017

Citation: Dalia S Ashour., et al. “Copro- Diagnosis of Early Trichinella spiralis Infection in Experimental Animals”. Acta Scientific Microbiology 1.1 (2018).

Abstract

   The early phase of trichinellosis is difficult to diagnose due to the non-specificity of the signs and symptoms that may be mis - diagnosed as alimentary intoxication or enterobacterial infection. Therefore, it is important to study early diagnostic methods for Trichinella spiralis infection to allow medical intervention at early stages of the disease. Coproantigen has become an important alternative method for the diagnosis of intestinal infections caused by either protozoa or helminths. To investigate the sensitivity of coproantigen detection in comparison with other traditional serodiagnostics, the following techniques were performed on T. spiralis infected rats: examination of the stool for Trichinella adult or newborn larvae, detection of coproantigen by capture ELISA and PCR, ELISA for detection of Trichinella antibodies in serum and PCR for detection of Trichinella DNA in the serum. We found that Trichinel- la coproantigen by ELISA and PCR show positivity from 1^st day until 9^th day post infection (P.I.). PCR detected T. spiralis DNA in serum from 5^th day P.I.. Trichinella antibodies in sera detected by ELISA technique show positivity starting from 10^th day P.I. till the end of the experiment. No Trichinella adults or larvae were detected in stool. In conclusion, early diagnosis of T. spiralis showed higher sensitiv - ity by copro-PCR and copro-ELISA followed by detection of T. spiralis DNA in serum then detection of T. spiralis antibodies in serum.

Keywords: Trichinella spiralis; Coproantigen; Copro-ELISA; Copro-PCR

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Copyright: © 2018 Dalia S Ashour., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.