Abstract

  The antimicrobial activity of ginseng extract was investigated in-vitro against 24 clinical isolates of Porphyromonas gingivalis and Prevotella intermedia. To our knowledge, there is a lack of researches on the evaluation of the antimicrobial effectiveness of ginseng extract on periodontopathic bacteria. We also compared the in-vitro antibacterial action of ginseng extract to that of propolis extract by agar diffusion method. The MIC values of ginseng extract against Porphyromonas gingivalis and Prevotella intermedia were 512 and 1024 mg/ml, respectively. While the MIC obtained for propolis extract were 256 and 512 mg/ml against Porphyromonas gingivalis and Prevotella intermedia, respectively. Moreover, the statistical analysis for the effect of ginseng and propolis extracts on Porphyromonas gingivalis and Prevotella intermedia using student paired t-test revealed a significant difference between the two groups (p < 0.001). However, the anti-Porphyromonas gingivalis and anti-Prevotella intermedia mean activities of ginseng extract were lower than that of propolis extract by 1.32 and 0.17 AU, respectively. The results of the current study support the usage of ginseng extract as an adjuvant to periodontal therapy after proper in-vivo studies are performed.

Keywords: Prevotella intermedia; Porphyromonas gingivalis; Propolis Extract; Ginseng Extract; Antibacterial

References

1. Hernandez M., et al. “Host-pathogen interactions in progressive chronic periodontitis”. Journal of Dental Research10 (2011): 1164-1170.
2. Zambon JJ. “Periodontal diseases: microbial factors”. Annals of Periodontology1 (1996): 879-925.
3. Slots J. “Systemic antibiotics in periodontics”. Journal of Periodontology 67 (1996): 831-838.
4. Lotfy WA., et al. “Production of di- (2-ethylhexyl) phthalate by Bacillus subtilis AD35: Isolation, purification, characterization and biological activities”. Microbial Pathogenesis 124 (2018): 89-100.
5. Heta S and I Robo. “The side effects of the most commonly used group of antibiotics in periodontal treatments”. Medical Sciences1 (2018): 6.
6. Santos FA., et al. “Susceptibility of Prevotella intermedia/Prevotella nigrescens (and Porphyromonas gingivalis) to propolis (bee glue) and other antimicrobial agents”. Anaerobe1 (2002): 9-15.
7. Sforcin JM. “Biological properties and therapeutic applications of propolis”. Phytotherapy Research6 (2016): 894-905.
8. Bankova VS., et al. “Propolis: recent advances in chemistry and plant origin”. Apidologie 1 (2000): 3-15.
9. Yoshimasu Y., et al. “Rapid bactericidal action of propolis against Porphyromonas gingivalis”. Journal of Dental Research8 (2018): 928-936.
10. Choi YH., et al. “Antibacterial and antioxidative activity of roasted coffee and red ginseng mixture extracts”. Journal of the Korean Society of Food Science and Nutrition3 (2012): 320-326.
11. Eom, S.J., et al. “Antibacterial effects against various foodborne pathogens and sensory properties of yogurt supplemented with panax ginseng marc extract”. Korean Journal for Food Science of Animal Resources5 (2017): 787.
12. Lee KA., et al. “Antibacterial activity of Ginseng (Panax ginseng CA Meyer) stems–leaves extract produced by subcritical water extraction”. International Journal of Food Science and Technology5 (2013): 947-953.
13. Singariya P., et al. “Evolution of Indian ginseng against different bacteria and fungi”. Asian Journal of Pharmaceutical and Clinical Research 5 (2012): 145-148.
14. Xu LL., et al. “Comparative research of chemical constituents, antifungal and antitumor properties of ether extracts of Panax ginseng and its endophytic fungus”. Phytomedicine 16 (2009): 609-616.
15. Son HJ., et al. “Antibacterial Activities of Et-OH Extract from Extruded White Ginseng on Tooth Decay Bacteria”. Journal of the Korean Society of Food Science and Nutrition7 (2009): 951-957.
16. Sun S., et al. “Effects of steaming the root of Panax notoginseng on chemical composition and anticancer activities”. Food Chemistry2 (2010): 307-314.
17. Doan N., et al. “Proficiencies of three anaerobic culture systems for recovering periodontal pathogenic bacteria”. Journal of Clinical Microbiology1 (1999): 171-174.
18. MacFaddin J. “Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria”. Williams and Wilkins, Baltimore Disclaimer: User must ensure suitability of the product (s) in their application prior to use. 1 (1985).
19. Jandik KA., et al. “Invasive differences among Porphyromonas gingivalis strains from healthy and diseased periodontal sites”. Journal of Periodontal Research5 (2008): 524-530.
20. Slots J and H Reynolds. “Long-wave UV light fluorescence for identification of black-pigmented Bacteroides spp”. Journal of Clinical Microbiology6 (1982): 1148-1151.
21. Randall LP., et al. “Evaluation of MALDI-ToF as a method for the identification of bacteria in the veterinary diagnostic laboratory”. Research in Veterinary Science 101 (2015): 42-49.
22. Atlas RM. Principles of microbiology/by Ronald M. Atlas. (1997).
23. Matar MA and WA Lotfy. “A Comparative Study of the Antimicrobial Activity of Cinnamomum zeylanicum and Thymus vulgaris Essential Oils with Chlorhexidine on Salivary Streptococcus mutans and Candida albicans”. Research and Reviews: A Journal of Dentistry1 (2019): 1-7.
24. Herrero ER., et al. “Dysbiosis by neutralizing commensal mediated inhibition of pathobionts”. Scientific Reports 6 (2016): 38179.
25. Lee JH., et al. “In vitro anti-adhesive activity of an acidic polysaccharide from Panax ginseng on Porphyromonas gingivalis binding to erythrocytes”. Planta Medica 70 (2004): 566-569.

Citation

Citation: Heba A Shawky and Walid A Lotfy. “A Comparison between the Antibacterial Activity of Propolis and Ginseng Extracts on Porphyromonas gingivalis and Prevotella intermedia Isolated from Patients with Chronic Periodontitis". Acta Scientific Dental Sciences 5.3 (2021): 82-87.

Copyright

Copyright: © 2020 Heba A Shawky and Walid A Lotfy. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.