Acta Scientific Dental Sciences (ISSN: 2581-4893)

Research Article Volume 4 Issue 10

A Comparative Evaluation of the Effectiveness of Saline, Chlorhexidine and Ozone Irrigating Solutions on Microorganisms in the Root Canal - An In Vivo Study

Alan Winston David1, Sarvepalli Venkata Satish2*, Sudhir Dole3, Suresh Kumar Kovvuru4 and Basavana Gowda5

1Postgraduate Student, Department of Conservative Dentistry and Endodontics, Navodaya Dental College, Navodaya Nagar, Raichur, India
2Professor and Head of the Department, Department of Conservative Dentistry and Endodontics, Navodaya Dental College, Navodaya Nagar, Raichur, India
3Consultant Dentist for Ozone Therapy and Trainer, Mumbai, India
4Professor, Department of Conservative Dentistry and Endodontics, Navodaya Dental College, Navodaya Nagar, Raichur, India
5Reader, Department of Conservative Dentistry and Endodontics, Navodaya Dental College, Navodaya Nagar, Raichur, India

*Corresponding Author: Sarvepalli Venkata Satish, Professor and Head of the Department, Department of Conservative Dentistry and Endodontics, Navodaya Dental College, Navodaya Nagar, Raichur, India.

Received: August 25, 2020; Published: September 28, 2020

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Abstract

Background: To evaluate the effectiveness of normal saline, 2% Chlorhexidine solution and Ozone oil on microorganisms in the root canal.

Materials and Methods: This study was carried out on 45 patients, out of which 60 anterior fractured teeth were selected which are asymptomatic, non-vital and necrotic in nature. Teeth were randomly divided into three groups of 20 each depending upon the type of irrigants used i.e. Saline, Chlorhexidine and Ozone. After access opening, two microbial samples were taken from each tooth: a) Pre- irrigation i.e. sample after pulp extirpation and before irrigation, b) Post-irrigation i.e. sample after irrigation, which were stored in sterile containers and immediately transferred to microbiological laboratory for incubation aerobically at 37°C for 24 hours. Then, the samples were streaked on blood agar culture media and incubated for 48 hours. After 48 hours, the colony forming units were counted using a colony counter. Statistical analysis was carried out using Kruskal-Wallis test, Wilcoxson signed rank test and Mann-Whitney test.

Results: On intra group comparison, highly significant differences in the colony forming units were found between pre- and post-irrigated samples for all the three irrigants used i.e. saline, chlorhexidine and ozone. On inter group comparison, statistical difference was found between saline (group 1) and chlorhexidine (group 2), saline (group 1) and Ozone oil (group 3), chlorhexidine (group 2) and Ozone oil (group 3).

Conclusion: The result of this study shows that Ozone oil irrigating solution can be used as an irrigating solution and its antibacterial action is effective when it is compared with 2% chlorhexidine and saline.

Keywords: Saline; Chlorhexidine; Ozone Oil; Non-Vital Teeth

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References

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Citation

Citation: Sarvepalli Venkata Satish., et al. “A Comparative Evaluation of the Effectiveness of Saline, Chlorhexidine and Ozone Irrigating Solutions on Microorganisms in the Root Canal - An In Vivo Study". Acta Scientific Dental Sciences 4.10 (2020): 76-81.



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